LOCUS       pMCS-Gaussia-Dur        4885 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Secreted Gaussia-Dura luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Gaussia-Dura Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4885)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4885)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..603
                     /label=Gaussia-Dura Luc
                     /note="enhanced variant of secreted Gaussia luciferase"
     polyA_signal    629..740
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        863..1192
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1240..1287
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1306..1902
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    2035..2168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2212..3069)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3333..3921
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4191..4207
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4663..4679
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    4794..4885
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccgccaccat gggagtcaaa
       61 gttctgtttg ccctgatctg catcgctgtg gccgaggcca agcccaccga gaacaacgaa
      121 gacttcaaca tcgtggccgt ggccagcaac ttcgcgacca cggatctcga tgctgaccgc
      181 gggaagttgc ccggcaagaa gctgccgctg gaggtgctca aagagttgga agccaatgcc
      241 cggaaagctg gctgcaccag gggctgtctg atctgcctgt cccacatcaa gtgcacgccc
      301 aagatgaaga agttcatccc aggacgctgc cacacctacg aaggcgacaa agagtccgca
      361 cagggcggca taggcgaggc gatcgtcgac attcctgaga ttcctgggtt caaggacttg
      421 gagcccttgg agcagttcat cgcacaggtc gatctgtgtg tggactgcac aactggctgc
      481 ctcaaagggc ttgccaacgt gcagtgttct gacctgctca agaagtggct gccgcaacgc
      541 tgtgcgacct ttgccagcaa gatccagggc caggtggaca agatcaaggg ggccggtggt
      601 gactaagcgg ccgcaaaatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      661 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      721 taaaatgagg aaattgcatc acaacactca accctatctc ggtctattct tttgatttat
      781 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      841 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      901 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      961 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1021 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     1081 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     1141 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     1201 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     1261 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatgac cgagtacaag
     1321 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     1381 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     1441 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     1501 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     1561 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     1621 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     1681 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     1741 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac atccgcgccc
     1801 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     1861 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacacgt gctacgagat
     1921 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     1981 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     2041 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     2101 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     2161 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcatt accaatgctt
     2221 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2281 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gcgctgcgat
     2341 gataccgcga gaaccacgct caccggctcc ggatttatca gcaataaacc agccagccgg
     2401 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     2461 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     2521 cgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     2581 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     2641 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     2701 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     2761 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     2821 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     2881 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     2941 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3001 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3061 aatactcata ttcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3121 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggtca gtgttacaac
     3181 caattaacca attctgaaca ttatcgcgag cccatttata cctgaatatg gctcataaca
     3241 ccccttgctc atgaccaaaa tcccttaacg tgagttacgc gcgcgtcgtt ccactgagcg
     3301 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3361 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3421 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3481 cttctagtgt agccgtagtt agcccaccac ttcaagaact ctgtagcacc gcctacatac
     3541 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3601 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3661 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3721 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3781 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3841 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3901 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3961 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     4021 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     4081 tcagtgagcg aggaagcgga aggcgagagt agggaactgc caggcatcaa actaagcaga
     4141 aggcccctga cggatggcct ttttgcgttt ctacaaactc tttctgtgtt gtaaaacgac
     4201 ggccagtctt aagctcgggc cccctgggcg gttctgataa cgagtaatcg ttaatccgca
     4261 aataacgtaa aaacccgctt cggcgggttt ttttatgggg ggagtttagg gaaagagcat
     4321 ttgtcagaat atttaagggc gcctgtcact ttgcttgata tatgagaatt atttaacctt
     4381 ataaatgaga aaaaagcaac gcactttaaa taagatacgt tgctttttcg attgatgaac
     4441 acctataatt aaactattca tctattattt atgatttttt gtatatacaa tatttctagt
     4501 ttgttaaaga gaattaagaa aataaatctc gaaaataata aagggaaaat cagtttttga
     4561 tatcaaaatt atacatgtca acgataatac aaaatataat acaaactata agatgttatc
     4621 agtatttatt atcatttaga ataaattttg tgtcgccctt aattgtgagc ggataacaat
     4681 tacgagcttc atgcacagtg gcgttgacat tgattattga ctagcatgtt ctttcctgcg
     4741 ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt aataacatac
     4801 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     4861 agtgcaggtg ccagaacatt tctct
//
