pMCS-Red Firefly Luc vector (V011467)

MSDS

Basic Vector Information

Vector Name:: pMCS-Red Firefly Luc

Antibiotic Resistance:: Ampicillin

Length:: 5977 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Thermo Scientific (Pierce)

Copy Number:: High copy number

Promoter:: EM7

pMCS-Red Firefly Luc vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMCS-Red Firefly Luc vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMCS-Red_Firefly        5977 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Intracellular red firefly luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Red Firefly Luc.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..1692
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     polyA_signal    1721..1832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1955..2284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2332..2379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2398..2994
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3127..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3304..4161)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4425..5013
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5283..5299
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5755..5771
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5886..5977
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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