LOCUS       pMCS-Red_Firefly        5977 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Intracellular red firefly luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Red Firefly Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5977)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5977)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5977
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..1692
                     /label=Red Firefly Luciferase
                     /note="intracellular red firefly luciferase"
     polyA_signal    1721..1832
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1955..2284
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2332..2379
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             2398..2994
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    3127..3260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3304..4161)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4425..5013
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5283..5299
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5755..5771
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5886..5977
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccgccaccat ggaaaatatg
       61 gaaaacgacg agaacatcgt ggtgggcccc aagcccttct accccatcga ggaaggcagc
      121 gccggcaccc agctgcggaa gtacatggaa agatacgcca agctgggcgc cattgccttc
      181 accaacgccg tgaccggcgt ggactacagc tacgccgagt acctggaaaa gagctgctgc
      241 ctgggcaagg ctctgcagaa ctacggcctg gtggtggacg gccggatcgc cctgtgcagc
      301 gagaactgcg aggaattctt catccccgtg atcgccggcc tgttcatcgg cgtgggcgtg
      361 gctcccacca acgagatcta caccctgcgg gagctggtgc acagcctggg catcagcaag
      421 cccaccatcg tgttcagcag caagaagggc ctggacaaag tcatcaccgt gcagaaaacc
      481 gtgaccacca tcaagaccat cgtgatcctg gacagcaagg tggactaccg gggctaccag
      541 tgcctggaca ccttcatcaa gcggaacacc ccccctggct tccaggccag cagcttcaag
      601 accgtggagg tggaccggaa agaacaggtg gccctgatca tgaacagcag cggcagcacc
      661 ggcctgccca agggcgtgca gctgacccac gagaacaccg tgacccggtt cagccacgcc
      721 agggacccca tctacggcaa ccaggtgtcc cccggcaccg ccgtgctgac cgtggtgccc
      781 ttccaccacg gcttcggcat gttcaccacc ctgggctacc tgatctgcgg cttccgggtg
      841 gtgatgctga ccaagttcga cgaggaaacc ttcctgaaaa ccctgcagga ctacaagtgc
      901 acctacgtga ttctggtgcc caccctgttc gccatcctga acaagagcga gctgctgaac
      961 aagtacgacc tgagcaacct ggtggagatc gccagcggcg gagcccccct gagcaaagaa
     1021 gtgggagagg ccgtcgccag gcggttcaat ctgcccggcg tgcggcaggg ctacggcctg
     1081 accgagacaa ccagcgccat catcatcacc cccgagggcg acgacaagcc tggagccagc
     1141 ggcaaggtgg tgcccctgtt caaggccaaa gtgatcgacc tggacaccaa gaagagcctg
     1201 ggccccaaca gacggggcga agtgtgcgtg aagggcccca tgctgatgaa gggctacgtg
     1261 aacaaccccg aggccaccaa agagctgatc gacgaagagg gctggctgca caccggcgac
     1321 atcggctact acgacgaaga gaagcacttc ttcatcgtgg accggctgaa gagcctgatc
     1381 aagtacaagg gctatcaggt gccccctgcc gagctggaaa gcgtcctgct gcagcacccc
     1441 agcatcttcg acgccggcgt ggccggggtg ccagatcctg tggccggcga gctgcctggc
     1501 gccgtggtgg tgctggaatc cggcaagaac atgaccgaga aagaagtgat ggactacgtc
     1561 gccagccagg tgtccaacgc caagcggctg agaggcggcg tgagattcgt ggacgaagtg
     1621 ccaaagggcc tgaccggcaa gatcgacggc agggccatcc gggagatcct gaagaaaccc
     1681 gtggccaaga tgtgatgagc ggccgcaaaa tcagcctcga ctgtgccttc tagttgccag
     1741 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     1801 gtcctttcct aataaaatga ggaaattgca tcacaacact caaccctatc tcggtctatt
     1861 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     1921 aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc
     1981 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
     2041 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2101 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     2161 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     2221 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     2281 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat
     2341 taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg
     2401 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     2461 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     2521 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     2581 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     2641 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     2701 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     2761 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     2821 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     2881 acatccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     2941 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacac
     3001 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     3061 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     3121 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     3181 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     3241 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     3301 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     3361 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     3421 cagcgctgcg atgataccgc gagaaccacg ctcaccggct ccggatttat cagcaataaa
     3481 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     3541 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     3601 cgttgttgcc atcgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     3661 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     3721 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     3781 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     3841 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     3901 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3961 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     4021 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     4081 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     4141 acggaaatgt tgaatactca tattcttcct ttttcaatat tattgaagca tttatcaggg
     4201 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     4261 cagtgttaca accaattaac caattctgaa cattatcgcg agcccattta tacctgaata
     4321 tggctcataa caccccttgc tcatgaccaa aatcccttaa cgtgagttac gcgcgcgtcg
     4381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     4561 ccaaatactg ttcttctagt gtagccgtag ttagcccacc acttcaagaa ctctgtagca
     4621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     4681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     4741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     4801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     4861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     4921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     4981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5161 gagcgcagcg agtcagtgag cgaggaagcg gaaggcgaga gtagggaact gccaggcatc
     5221 aaactaagca gaaggcccct gacggatggc ctttttgcgt ttctacaaac tctttctgtg
     5281 ttgtaaaacg acggccagtc ttaagctcgg gccccctggg cggttctgat aacgagtaat
     5341 cgttaatccg caaataacgt aaaaacccgc ttcggcgggt ttttttatgg ggggagttta
     5401 gggaaagagc atttgtcaga atatttaagg gcgcctgtca ctttgcttga tatatgagaa
     5461 ttatttaacc ttataaatga gaaaaaagca acgcacttta aataagatac gttgcttttt
     5521 cgattgatga acacctataa ttaaactatt catctattat ttatgatttt ttgtatatac
     5581 aatatttcta gtttgttaaa gagaattaag aaaataaatc tcgaaaataa taaagggaaa
     5641 atcagttttt gatatcaaaa ttatacatgt caacgataat acaaaatata atacaaacta
     5701 taagatgtta tcagtattta ttatcattta gaataaattt tgtgtcgccc ttaattgtga
     5761 gcggataaca attacgagct tcatgcacag tggcgttgac attgattatt gactagcatg
     5821 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcattagtta
     5881 ttaataacat acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct
     5941 gtccccagtg caagtgcagg tgccagaaca tttctct
//
