pNL2.1[Nluc/Hygro] vector (V011454)

LOCUS       pNL2.1[Nluc_Hygr        4709 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL2.1[Nluc Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..612
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(664..785)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        979..1336
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1367..2401
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2428..2476
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2803..3391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3594..4451)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4556..4604
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4618..4709
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"