LOCUS       pNL2.1[Nluc_Hygr        4709 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL2.1[Nluc Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4709)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4709
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..612
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(664..785)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        979..1336
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1367..2401
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2428..2476
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2803..3391)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3594..4451)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4556..4604
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4618..4709
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggtcttcac actcgaagat
      121 ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct tgaacaggga
      181 ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca aaggattgtc
      241 ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta tgaaggtctg
      301 agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc tgtggatgat
      361 catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt tacgccgaac
      421 atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg caaaaagatc
      481 actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct gatcaacccc
      541 gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg gctgtgcgaa
      601 cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
      661 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
      721 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
      781 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
      841 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
      901 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
      961 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     1021 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     1081 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     1141 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     1201 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     1261 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     1321 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga agaagcccga
     1381 actcaccgct accagcgttg aaaaatttct catcgagaag ttcgacagtg tgagcgacct
     1441 gatgcagttg tcggagggcg aagagagccg agccttcagc ttcgatgtcg gcggacgcgg
     1501 ctatgtactg cgggtgaata gctgcgctga tggcttctac aaagaccgct acgtgtaccg
     1561 ccacttcgcc agcgctgcac tacccatccc cgaagtgttg gacatcggcg agttcagcga
     1621 gagcctgaca tactgcatca gtagacgcgc ccaaggcgtt actctccaag acctccccga
     1681 aacagagctg cctgctgtgt tacagcctgt cgccgaagct atggatgcta ttgccgccgc
     1741 cgacctcagt caaaccagcg gcttcggccc attcgggccc caaggcatcg gccagtacac
     1801 aacctggcgg gatttcattt gcgccattgc tgatccccat gtctaccact ggcagaccgt
     1861 gatggacgac accgtgtccg ccagcgtagc tcaagccctg gacgaactga tgctgtgggc
     1921 cgaagactgt cccgaggtgc gccacctcgt ccatgccgac ttcggcagca acaacgtcct
     1981 gaccgacaac ggccgcatca ccgccgtaat cgactggtcc gaagctatgt tcggggacag
     2041 tcagtacgag gtggccaaca tcttcttctg gcggccctgg ctggcttgca tggagcagca
     2101 gactcgctac ttcgagcgcc ggcatcccga gctggccggc agccctcgtc tgcgagccta
     2161 catgctgcgc atcggcctgg atcagctcta ccagagcctc gtggacggca acttcgacga
     2221 tgctgcctgg gctcaaggcc gctgcgatgc catcgtccgc agcggggccg gcaccgtcgg
     2281 tcgcacacaa atcgctcgcc ggagcgcagc cgtatggacc gacggctgcg tcgaggtgct
     2341 ggccgacagc ggcaaccgcc ggcccagtac acgaccgcgc gctaaggagg taggtcgagt
     2401 ttaaactcta gaaccggtca tggccgcaat aaaatatctt tattttcatt acatctgtgt
     2461 gttggttttt tgtgtgttcg aactagatgc tgtcgaccga tgcccttgag agccttcaac
     2521 ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact tatgactgtc
     2581 ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt cctcgctcac
     2641 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     2701 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     2761 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2821 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     2881 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     2941 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3001 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3061 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3121 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3181 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3241 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3301 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3361 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3421 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     3481 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     3541 tgagtaaact tggtctgaca gcggccgcaa atgctaaacc actgcagtgg ttaccagtgc
     3601 ttgatcagtg aggcaccgat ctcagcgatc tgcctatttc gttcgtccat agtggcctga
     3661 ctccccgtcg tgtagatcac tacgattcgt gagggcttac catcaggccc cagcgcagca
     3721 atgatgccgc gagagccgcg ttcaccggcc cccgatttgt cagcaatgaa ccagccagca
     3781 gggagggccg agcgaagaag tggtcctgct actttgtccg cctccatcca gtctatgagc
     3841 tgctgtcgtg atgctagagt aagaagttcg ccagtgagta gtttccgaag agttgtggcc
     3901 attgctactg gcatcgtggt atcacgctcg tcgttcggta tggcttcgtt caactctggt
     3961 tcccagcggt caagccgggt cacatgatca cccatattat gaagaaatgc agtcagctcc
     4021 ttagggcctc cgatcgttgt cagaagtaag ttggccgcgg tgttgtcgct catggtaatg
     4081 gcagcactac acaattctct taccgtcatg ccatccgtaa gatgcttttc cgtgaccggc
     4141 gagtactcaa ccaagtcgtt ttgtgagtag tgtatacggc gaccaagctg ctcttgcccg
     4201 gcgtctatac gggacaacac cgcgccacat agcagtactt tgaaagtgct catcatcggg
     4261 aatcgttctt cggggcggaa agactcaagg atcttgccgc tattgagatc cagttcgata
     4321 tagcccactc ttgcacccag ttgatcttca gcatctttta ctttcaccag cgtttcgggg
     4381 tgtgcaaaaa caggcaagca aaatgccgca aagaagggaa tgagtgcgac acgaaaatgt
     4441 tggatgctca tactcgtcct ttttcaatat tattgaagca tttatcaggg ttactagtac
     4501 gtctctcaag gataagtaag taatattaag gtacgggagg tattggacag gccgcaataa
     4561 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac
     4621 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag
     4681 tgcaagtgca ggtgccagaa catttctct
//
