Basic Vector Information
- Vector Name:
- pNL2.3[secNluc/Hygro]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 4793 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Copy Number:
- High copy number
- Promoter:
- SV40
pNL2.3[secNluc/Hygro] vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNL2.3[secNluc/Hygro] vector Sequence
LOCUS pNL2.3[secNluc_H 4793 bp DNA circular SYN 01-JAN-1980 DEFINITION Promoterless vector for measuring the activity of promoter and enhancer sequences with a secreted NanoLuc(R) luciferase assay. ACCESSION . VERSION . KEYWORDS pNL2.3[secNluc Hygro]. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4793) AUTHORS Promega TITLE Direct Submission REFERENCE 2 (bases 1 to 4793) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..4793 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..70 /label=MCS /note="MCS" /note="multiple cloning site" CDS 187..696 /label=Nluc /note="NanoLuc(R) luciferase" polyA_signal complement(748..869) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" promoter 1063..1420 /label=SV40 promoter /note="SV40 enhancer and early promoter" CDS 1451..2485 /label=HygR /note="hygromycin B phosphotransferase" polyA_signal 2512..2560 /label=poly(A) signal /note="synthetic polyadenylation signal" rep_origin complement(2887..3475) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3678..4535) /label=AmpR /note="beta-lactamase" polyA_signal 4640..4688 /label=synthetic polyadenylation signal /note="synthetic polyadenylation signal" misc_feature 4702..4793 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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