LOCUS       pNL2.3[secNluc_H        4793 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector for measuring the activity of promoter and 
            enhancer sequences with a secreted NanoLuc(R) luciferase assay.
ACCESSION   .
VERSION     .
KEYWORDS    pNL2.3[secNluc Hygro].
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             187..696
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(748..869)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1063..1420
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1451..2485
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2512..2560
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(2887..3475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3678..4535)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4640..4688
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4702..4793
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tgaactcctt ctccacaagc
      121 gccttcggtc cagttgcctt ctccctgggc ctgctcctgg tgttgcctgc tgccttccct
      181 gccccagtct tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac
      241 ctggaccaag tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc
      301 gtaactccga tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat
      361 gtcatcatcc cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt
      421 aaggtggtgt accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg
      481 gtaatcgacg gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc
      541 gccgtgttcg acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt
      601 atcgacgagc gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga
      661 gtgaccggct ggcggctgtg cgaacgcatt ctggcgtaag gccgcgactc tagagtcggg
      721 gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga caaaccacaa
      781 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg
      841 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
      901 aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac aaatgtggta
      961 aaatcgataa ggatccgttt gcgtattggg cgctcttccg ctgatctgcg cagcaccatg
     1021 gcctgaaata acctctgaaa gaggaacttg gttagctacc ttctgaggcg gaaagaacca
     1081 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     1141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     1201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     1261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     1321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     1381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcgatt cttctgacac
     1441 tagcgccacc atgaagaagc ccgaactcac cgctaccagc gttgaaaaat ttctcatcga
     1501 gaagttcgac agtgtgagcg acctgatgca gttgtcggag ggcgaagaga gccgagcctt
     1561 cagcttcgat gtcggcggac gcggctatgt actgcgggtg aatagctgcg ctgatggctt
     1621 ctacaaagac cgctacgtgt accgccactt cgccagcgct gcactaccca tccccgaagt
     1681 gttggacatc ggcgagttca gcgagagcct gacatactgc atcagtagac gcgcccaagg
     1741 cgttactctc caagacctcc ccgaaacaga gctgcctgct gtgttacagc ctgtcgccga
     1801 agctatggat gctattgccg ccgccgacct cagtcaaacc agcggcttcg gcccattcgg
     1861 gccccaaggc atcggccagt acacaacctg gcgggatttc atttgcgcca ttgctgatcc
     1921 ccatgtctac cactggcaga ccgtgatgga cgacaccgtg tccgccagcg tagctcaagc
     1981 cctggacgaa ctgatgctgt gggccgaaga ctgtcccgag gtgcgccacc tcgtccatgc
     2041 cgacttcggc agcaacaacg tcctgaccga caacggccgc atcaccgccg taatcgactg
     2101 gtccgaagct atgttcgggg acagtcagta cgaggtggcc aacatcttct tctggcggcc
     2161 ctggctggct tgcatggagc agcagactcg ctacttcgag cgccggcatc ccgagctggc
     2221 cggcagccct cgtctgcgag cctacatgct gcgcatcggc ctggatcagc tctaccagag
     2281 cctcgtggac ggcaacttcg acgatgctgc ctgggctcaa ggccgctgcg atgccatcgt
     2341 ccgcagcggg gccggcaccg tcggtcgcac acaaatcgct cgccggagcg cagccgtatg
     2401 gaccgacggc tgcgtcgagg tgctggccga cagcggcaac cgccggccca gtacacgacc
     2461 gcgcgctaag gaggtaggtc gagtttaaac tctagaaccg gtcatggccg caataaaata
     2521 tctttatttt cattacatct gtgtgttggt tttttgtgtg ttcgaactag atgctgtcga
     2581 ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     2641 atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     2701 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     2761 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     2821 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     2881 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     2941 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     3001 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     3061 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     3121 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     3181 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     3241 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     3301 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     3361 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     3421 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     3481 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     3541 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     3601 ttttaaatca atctaaagta tatatgagta aacttggtct gacagcggcc gcaaatgcta
     3661 aaccactgca gtggttacca gtgcttgatc agtgaggcac cgatctcagc gatctgccta
     3721 tttcgttcgt ccatagtggc ctgactcccc gtcgtgtaga tcactacgat tcgtgagggc
     3781 ttaccatcag gccccagcgc agcaatgatg ccgcgagagc cgcgttcacc ggcccccgat
     3841 ttgtcagcaa tgaaccagcc agcagggagg gccgagcgaa gaagtggtcc tgctactttg
     3901 tccgcctcca tccagtctat gagctgctgt cgtgatgcta gagtaagaag ttcgccagtg
     3961 agtagtttcc gaagagttgt ggccattgct actggcatcg tggtatcacg ctcgtcgttc
     4021 ggtatggctt cgttcaactc tggttcccag cggtcaagcc gggtcacatg atcacccata
     4081 ttatgaagaa atgcagtcag ctccttaggg cctccgatcg ttgtcagaag taagttggcc
     4141 gcggtgttgt cgctcatggt aatggcagca ctacacaatt ctcttaccgt catgccatcc
     4201 gtaagatgct tttccgtgac cggcgagtac tcaaccaagt cgttttgtga gtagtgtata
     4261 cggcgaccaa gctgctcttg cccggcgtct atacgggaca acaccgcgcc acatagcagt
     4321 actttgaaag tgctcatcat cgggaatcgt tcttcggggc ggaaagactc aaggatcttg
     4381 ccgctattga gatccagttc gatatagccc actcttgcac ccagttgatc ttcagcatct
     4441 tttactttca ccagcgtttc ggggtgtgca aaaacaggca agcaaaatgc cgcaaagaag
     4501 ggaatgagtg cgacacgaaa atgttggatg ctcatactcg tcctttttca atattattga
     4561 agcatttatc agggttacta gtacgtctct caaggataag taagtaatat taaggtacgg
     4621 gaggtattgg acaggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     4681 tttgtgtgaa tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa
     4741 actagcaaaa taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct
//
