pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] vector (V011450)

MSDS

Basic Vector Information

Vector Name:: pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro]

Antibiotic Resistance:: Ampicillin

Length:: 4912 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Promega

Copy Number:: High copy number

Promoter:: SV40

pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pNL3.2.NF-κB-RE[NlucP/NF-κB-RE/Hygro] vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pNL3.2.NF-kB-RE[        4912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and NF-kappa-B response element for 
            studying cell signaling using destabilized NanoLuc(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro].
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..84
                     /label=NF-kappa-B binding site
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B RE"
                     /note="NF-kappa-B response element (5 copies)"
     promoter        116..147
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             180..692
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             696..815
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(867..988)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1182..1539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1570..2604
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2631..2679
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3006..3594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3797..4654)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4759..4807
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"

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