LOCUS       pNL3.2.NF-kB-RE[        4912 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and NF-kappa-B response element for 
            studying cell signaling using destabilized NanoLuc(R) luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.2.NF-kB-RE[NlucP NF-kB-RE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4912)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    33..84
                     /label=NF-kappa-B binding site
                     /bound_moiety="NF-kappa-B"
                     /note="NF-kappa-B RE"
                     /note="NF-kappa-B response element (5 copies)"
     promoter        116..147
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             180..692
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             696..815
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(867..988)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1182..1539
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1570..2604
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    2631..2679
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3006..3594)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3797..4654)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    4759..4807
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4821..4912
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcgggaattt ccggggactt tccgggaatt
       61 tccggggact ttccgggaat ttccagatct ggcctcggcg gccaagctta gacactagag
      121 ggtatataat ggaagctcga cttccagctt ggcaatccgg tactgttggt aaagccacca
      181 tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg
      241 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa
      301 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca
      361 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg
      421 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa
      481 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg
      541 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
      601 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga
      661 ccggctggcg gctgtgcgaa cgcattctgg cgaattctca cggctttccg cctgaggttg
      721 aagagcaagc cgccggtaca ttgcctatgt cctgcgcaca agaaagcggt atggaccggc
      781 acccagccgc ttgtgcttca gctcgcatca acgtctaagg ccgcgactct agagtcgggg
      841 cggccggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
      901 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
      961 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     1021 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
     1081 aatcgataag gatccgtttg cgtattgggc gctcttccgc tgatctgcgc agcaccatgg
     1141 cctgaaataa cctctgaaag aggaacttgg ttagctacct tctgaggcgg aaagaaccag
     1201 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     1261 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     1321 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     1381 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     1441 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     1501 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcgattc ttctgacact
     1561 agcgccacca tgaagaagcc cgaactcacc gctaccagcg ttgaaaaatt tctcatcgag
     1621 aagttcgaca gtgtgagcga cctgatgcag ttgtcggagg gcgaagagag ccgagccttc
     1681 agcttcgatg tcggcggacg cggctatgta ctgcgggtga atagctgcgc tgatggcttc
     1741 tacaaagacc gctacgtgta ccgccacttc gccagcgctg cactacccat ccccgaagtg
     1801 ttggacatcg gcgagttcag cgagagcctg acatactgca tcagtagacg cgcccaaggc
     1861 gttactctcc aagacctccc cgaaacagag ctgcctgctg tgttacagcc tgtcgccgaa
     1921 gctatggatg ctattgccgc cgccgacctc agtcaaacca gcggcttcgg cccattcggg
     1981 ccccaaggca tcggccagta cacaacctgg cgggatttca tttgcgccat tgctgatccc
     2041 catgtctacc actggcagac cgtgatggac gacaccgtgt ccgccagcgt agctcaagcc
     2101 ctggacgaac tgatgctgtg ggccgaagac tgtcccgagg tgcgccacct cgtccatgcc
     2161 gacttcggca gcaacaacgt cctgaccgac aacggccgca tcaccgccgt aatcgactgg
     2221 tccgaagcta tgttcgggga cagtcagtac gaggtggcca acatcttctt ctggcggccc
     2281 tggctggctt gcatggagca gcagactcgc tacttcgagc gccggcatcc cgagctggcc
     2341 ggcagccctc gtctgcgagc ctacatgctg cgcatcggcc tggatcagct ctaccagagc
     2401 ctcgtggacg gcaacttcga cgatgctgcc tgggctcaag gccgctgcga tgccatcgtc
     2461 cgcagcgggg ccggcaccgt cggtcgcaca caaatcgctc gccggagcgc agccgtatgg
     2521 accgacggct gcgtcgaggt gctggccgac agcggcaacc gccggcccag tacacgaccg
     2581 cgcgctaagg aggtaggtcg agtttaaact ctagaaccgg tcatggccgc aataaaatat
     2641 ctttattttc attacatctg tgtgttggtt ttttgtgtgt tcgaactaga tgctgtcgac
     2701 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2761 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2821 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2881 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2941 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3001 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3061 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3121 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3181 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3241 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3301 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3361 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3421 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3481 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3541 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3601 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3661 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3721 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagcggccg caaatgctaa
     3781 accactgcag tggttaccag tgcttgatca gtgaggcacc gatctcagcg atctgcctat
     3841 ttcgttcgtc catagtggcc tgactccccg tcgtgtagat cactacgatt cgtgagggct
     3901 taccatcagg ccccagcgca gcaatgatgc cgcgagagcc gcgttcaccg gcccccgatt
     3961 tgtcagcaat gaaccagcca gcagggaggg ccgagcgaag aagtggtcct gctactttgt
     4021 ccgcctccat ccagtctatg agctgctgtc gtgatgctag agtaagaagt tcgccagtga
     4081 gtagtttccg aagagttgtg gccattgcta ctggcatcgt ggtatcacgc tcgtcgttcg
     4141 gtatggcttc gttcaactct ggttcccagc ggtcaagccg ggtcacatga tcacccatat
     4201 tatgaagaaa tgcagtcagc tccttagggc ctccgatcgt tgtcagaagt aagttggccg
     4261 cggtgttgtc gctcatggta atggcagcac tacacaattc tcttaccgtc atgccatccg
     4321 taagatgctt ttccgtgacc ggcgagtact caaccaagtc gttttgtgag tagtgtatac
     4381 ggcgaccaag ctgctcttgc ccggcgtcta tacgggacaa caccgcgcca catagcagta
     4441 ctttgaaagt gctcatcatc gggaatcgtt cttcggggcg gaaagactca aggatcttgc
     4501 cgctattgag atccagttcg atatagccca ctcttgcacc cagttgatct tcagcatctt
     4561 ttactttcac cagcgtttcg gggtgtgcaa aaacaggcaa gcaaaatgcc gcaaagaagg
     4621 gaatgagtgc gacacgaaaa tgttggatgc tcatactcgt cctttttcaa tattattgaa
     4681 gcatttatca gggttactag tacgtctctc aaggataagt aagtaatatt aaggtacggg
     4741 aggtattgga caggccgcaa taaaatatct ttattttcat tacatctgtg tgttggtttt
     4801 ttgtgtgaat cgatagtact aacatacgct ctccatcaaa acaaaacgaa acaaaacaaa
     4861 ctagcaaaat aggctgtccc cagtgcaagt gcaggtgcca gaacatttct ct
//
