Basic Vector Information
- Vector Name:
- pNL3.2[NlucP/minP]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3274 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Copy Number:
- High copy number
- Promoter:
- minP
pNL3.2[NlucP/minP] vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNL3.2[NlucP/minP] vector Sequence
LOCUS pNL3.2[NlucP_min 3274 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector with a minimal promoter for measuring the activity of transcriptional response elements using destabilized NanoLuc(R) luciferase. ACCESSION . VERSION . KEYWORDS pNL3.2[NlucP minP]. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3274) AUTHORS Promega TITLE Direct Submission REFERENCE 2 (bases 1 to 3274) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3274 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..70 /label=MCS /note="MCS" /note="multiple cloning site" promoter 77..108 /label=minP /note="minimal TATA-box promoter with low basal activity" CDS 141..653 /label=Nluc /note="NanoLuc(R) luciferase" CDS 657..776 /label=hPEST /note="PEST degradation sequence from mouse ornithine decarboxylase" polyA_signal complement(828..949) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1368..1956) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2159..3016) /label=AmpR /note="beta-lactamase" polyA_signal 3121..3169 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 3183..3274 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
This page is informational only.