LOCUS       pNL3.2[NlucP_min        3274 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using destabilized NanoLuc(R) 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.2[NlucP minP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3274
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..653
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     CDS             657..776
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(828..949)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1368..1956)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2159..3016)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3121..3169
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3183..3274
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atggtcttca cactcgaaga tttcgttggg gactggcgac
      181 agacagccgg ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc
      241 agaatctcgg ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc
      301 tgaagatcga catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc
      361 agatcgaaaa aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc
      421 tgcactatgg cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac
      481 ggccgtatga aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt
      541 ggaacggcaa caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc
      601 gagtaaccat caacggagtg accggctggc ggctgtgcga acgcattctg gcgaattctc
      661 acggctttcc gcctgaggtt gaagagcaag ccgccggtac attgcctatg tcctgcgcac
      721 aagaaagcgg tatggaccgg cacccagccg cttgtgcttc agctcgcatc aacgtctaag
      781 gccgcgactc tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga
      841 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      901 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      961 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta
     1021 aaacctctac aaatgtggta aaatcgataa ggatccgtcg accgatgccc ttgagagcct
     1081 tcaacccagt cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga
     1141 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg
     1201 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     1261 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     1321 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     1381 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     1441 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     1501 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     1561 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     1621 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     1681 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     1741 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     1801 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     1861 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     1921 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     1981 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     2041 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     2101 atatatgagt aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc
     2161 agtgcttgat cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg
     2221 cctgactccc cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg
     2281 cagcaatgat gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc
     2341 cagcagggag ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta
     2401 tgagctgctg tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg
     2461 tggccattgc tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact
     2521 ctggttccca gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca
     2581 gctccttagg gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg
     2641 taatggcagc actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga
     2701 ccggcgagta ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt
     2761 gcccggcgtc tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca
     2821 tcgggaatcg ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt
     2881 cgatatagcc cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt
     2941 cggggtgtgc aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa
     3001 aatgttggat gctcatactc gtcctttttc aatattattg aagcatttat cagggttact
     3061 agtacgtctc tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc
     3121 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
     3181 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
     3241 cccagtgcaa gtgcaggtgc cagaacattt ctct
//
