Basic Vector Information
- Vector Name:
- pNL3.3[secNluc/minP]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3235 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Copy Number:
- High copy number
- Promoter:
- minP
pNL3.3[secNluc/minP] vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNL3.3[secNluc/minP] vector Sequence
LOCUS pNL3.3[secNluc_m 3235 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector with a minimal promoter for measuring the activity of transcriptional response elements using secreted NanoLuc(R) luciferase. ACCESSION . VERSION . KEYWORDS pNL3.3[secNluc minP]. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3235) AUTHORS Promega TITLE Direct Submission REFERENCE 2 (bases 1 to 3235) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3235 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..70 /label=MCS /note="MCS" /note="multiple cloning site" promoter 77..108 /label=minP /note="minimal TATA-box promoter with low basal activity" CDS 141..227 /codon_start=1 /product="secretion signal from interleukin 6" /label=secretion signal from interleukin 6 /note="IL-6 signal sequence" /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP" CDS 228..737 /label=Nluc /note="NanoLuc(R) luciferase" polyA_signal complement(789..910) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1329..1917) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2120..2977) /label=AmpR /note="beta-lactamase" polyA_signal 3082..3130 /label=poly(A) signal /note="synthetic polyadenylation signal" misc_feature 3144..3235 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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