LOCUS       pNL3.3[secNluc_m        3235 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter for measuring the activity of 
            transcriptional response elements using secreted NanoLuc(R) 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pNL3.3[secNluc minP]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        77..108
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             141..227
                     /codon_start=1
                     /product="secretion signal from interleukin 6"
                     /label=secretion signal from interleukin 6
                     /note="IL-6 signal sequence"
                     /translation="MNSFSTSAFGPVAFSLGLLLVLPAAFPAP"
     CDS             228..737
                     /label=Nluc
                     /note="NanoLuc(R) luciferase"
     polyA_signal    complement(789..910)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1329..1917)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2120..2977)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    3082..3130
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    3144..3235
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt agacactaga gggtatataa tggaagctcg acttccagct tggcaatccg
      121 gtactgttgg taaagccacc atgaactcct tctccacaag cgccttcggt ccagttgcct
      181 tctccctggg cctgctcctg gtgttgcctg ctgccttccc tgccccagtc ttcacactcg
      241 aagatttcgt tggggactgg cgacagacag ccggctacaa cctggaccaa gtccttgaac
      301 agggaggtgt gtccagtttg tttcagaatc tcggggtgtc cgtaactccg atccaaagga
      361 ttgtcctgag cggtgaaaat gggctgaaga tcgacatcca tgtcatcatc ccgtatgaag
      421 gtctgagcgg cgaccaaatg ggccagatcg aaaaaatttt taaggtggtg taccctgtgg
      481 atgatcatca ctttaaggtg atcctgcact atggcacact ggtaatcgac ggggttacgc
      541 cgaacatgat cgactatttc ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa
      601 agatcactgt aacagggacc ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca
      661 accccgacgg ctccctgctg ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt
      721 gcgaacgcat tctggcgtaa ggccgcgact ctagagtcgg ggcggccggc cgcttcgagc
      781 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
      841 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
      901 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
      961 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
     1021 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     1081 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     1141 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1201 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1261 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1321 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1381 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1441 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1501 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1561 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1621 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1681 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1741 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1801 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1861 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1921 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1981 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2041 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagcgg ccgcaaatgc
     2101 taaaccactg cagtggttac cagtgcttga tcagtgaggc accgatctca gcgatctgcc
     2161 tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta gatcactacg attcgtgagg
     2221 gcttaccatc aggccccagc gcagcaatga tgccgcgaga gccgcgttca ccggcccccg
     2281 atttgtcagc aatgaaccag ccagcaggga gggccgagcg aagaagtggt cctgctactt
     2341 tgtccgcctc catccagtct atgagctgct gtcgtgatgc tagagtaaga agttcgccag
     2401 tgagtagttt ccgaagagtt gtggccattg ctactggcat cgtggtatca cgctcgtcgt
     2461 tcggtatggc ttcgttcaac tctggttccc agcggtcaag ccgggtcaca tgatcaccca
     2521 tattatgaag aaatgcagtc agctccttag ggcctccgat cgttgtcaga agtaagttgg
     2581 ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa ttctcttacc gtcatgccat
     2641 ccgtaagatg cttttccgtg accggcgagt actcaaccaa gtcgttttgt gagtagtgta
     2701 tacggcgacc aagctgctct tgcccggcgt ctatacggga caacaccgcg ccacatagca
     2761 gtactttgaa agtgctcatc atcgggaatc gttcttcggg gcggaaagac tcaaggatct
     2821 tgccgctatt gagatccagt tcgatatagc ccactcttgc acccagttga tcttcagcat
     2881 cttttacttt caccagcgtt tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga
     2941 agggaatgag tgcgacacga aaatgttgga tgctcatact cgtccttttt caatattatt
     3001 gaagcattta tcagggttac tagtacgtct ctcaaggata agtaagtaat attaaggtac
     3061 gggaggtatt ggacaggccg caataaaata tctttatttt cattacatct gtgtgttggt
     3121 tttttgtgtg aatcgatagt actaacatac gctctccatc aaaacaaaac gaaacaaaac
     3181 aaactagcaa aataggctgt ccccagtgca agtgcaggtg ccagaacatt tctct
//
