pRL-null vector (V011446)

LOCUS       pRL-null.        3320 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless mammalian vector encoding wild-type Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pRL-null
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..78
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          104..236
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             309..1241
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     polyA_signal    complement(1275..1396)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1529..1633
                     /label=AmpR promoter
     CDS             1634..2491
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2665..3253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"