LOCUS       pRL-null.        3320 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless mammalian vector encoding wild-type Renilla luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pRL-null
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..78
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     intron          104..236
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             309..1241
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     polyA_signal    complement(1275..1396)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1529..1633
                     /label=AmpR promoter
     CDS             1634..2491
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2665..3253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctcgag ctctaagctt cacatatgca tgcactagtg gcgcctgtcg acgcgtagaa
       61 ttcacccggg tactgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      121 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      181 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      241 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
      301 tagccaccat gacttcgaaa gtttatgatc cagaacaaag gaaacggatg ataactggtc
      361 cgcagtggtg ggccagatgt aaacaaatga atgttcttga ttcatttatt aattattatg
      421 attcagaaaa acatgcagaa aatgctgtta tttttttaca tggtaacgcg gcctcttctt
      481 atttatggcg acatgttgtg ccacatattg agccagtagc gcggtgtatt ataccagacc
      541 ttattggtat gggcaaatca ggcaaatctg gtaatggttc ttataggtta cttgatcatt
      601 acaaatatct tactgcatgg tttgaacttc ttaatttacc aaagaagatc atttttgtcg
      661 gccatgattg gggtgcttgt ttggcatttc attatagcta tgagcatcaa gataagatca
      721 aagcaatagt tcacgctgaa agtgtagtag atgtgattga atcatgggat gaatggcctg
      781 atattgaaga agatattgcg ttgatcaaat ctgaagaagg agaaaaaatg gttttggaga
      841 ataacttctt cgtggaaacc atgttgccat caaaaatcat gagaaagtta gaaccagaag
      901 aatttgcagc atatcttgaa ccattcaaag agaaaggtga agttcgtcgt ccaacattat
      961 catggcctcg tgaaatcccg ttagtaaaag gtggtaaacc tgacgttgta caaattgtta
     1021 ggaattataa tgcttatcta cgtgcaagtg atgatttacc aaaaatgttt attgaatcgg
     1081 acccaggatt cttttccaat gctattgttg aaggtgccaa gaagtttcct aatactgaat
     1141 ttgtcaaagt aaaaggtctt catttttcgc aagaagatgc acctgatgaa atgggaaaat
     1201 atatcaaatc gttcgttgag cgagttctca aaaatgaaca ataattctag agcggccgct
     1261 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1321 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1381 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1441 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     1501 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3301 ttttgctcac atggctcgac
//
