pRham N-His Kan vector (V011380)

LOCUS       pRham_N-His_Kan.        2275 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for Expresso(R) cloning to add an N-terminal 6xHis tag, with 
            rhamnose-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    pRham N-His Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             153..175
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             183..185
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             186..203
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      235..282
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      380..409
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      431..1018
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1030..1057)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1084..1272)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(1276..2088)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2089..2179)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2180..2211)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"