LOCUS       pRham_N-His_Kan.        2275 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for Expresso(R) cloning to add an N-terminal 6xHis tag, with 
            rhamnose-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    pRham N-His Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             153..175
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             183..185
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             186..203
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      235..282
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      380..409
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      431..1018
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1030..1057)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1084..1272)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(1276..2088)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2089..2179)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2180..2211)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"
ORIGIN
        1 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
       61 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtag
      121 ggagaccaca acggtttccc tctagaaata attttgttta actataagaa ggagatatac
      181 atatgcatca tcaccaccat cactaataga gcggccgcca ccgctgagca ataactagca
      241 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
      301 tccgggtaac gaattcaagc ttgatatcat tcaggacgag cctcagactc cagcgtaact
      361 ggactgcaat caactcactg gctcaccttc acgggtgggc ctttcttcgg tagaaaatca
      421 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
      481 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaggt
      541 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      601 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      661 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      721 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      781 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      841 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      901 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      961 cctctgactt gagcatcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1021 cgccagcaac gcagaaaggc ccacccgaag gtgagccagg tgattacatt tgggccctca
     1081 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc
     1141 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt
     1201 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc
     1261 ctgtttggtc atttagaaaa actcatcgag catcaagtga aactgcaatt tattcatatc
     1321 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     1381 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     1441 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     1501 atgagtgacg actgaatccg gtgagaatgg caaaagctta tgcatttctt tccagacttg
     1561 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcaccaacca aaccgttatt
     1621 cattcgtgat tgcgcctgag cgagacgaaa tacgcgatcg ccgttaaaag gacaattaca
     1681 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     1741 tgaatcagga tattcttcta atacctggaa tgctgttttc cctgggatcg cagtggtgag
     1801 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     1861 cgtcagccag tttagcctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     1921 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     1981 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     2041 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcatag ctcctgaaaa
     2101 tctcgataac tcaaaaaata cgcccggtag tgatcttatt tcattatggt gaaagttgga
     2161 acctcttacg tgccgatcaa gtcaaaagcc tccggtcgga ggcttttgac tttctgctat
     2221 ggaggtcagg tatgatttaa atggtcagta ttgagcgata tctagagaat tcgtc
//
