pmRi-mCherry vector (V011099)

MSDS

Basic Vector Information

- - Vector Name:
  - pmRi-mCherry
- - Antibiotic Resistance:
  - Ampicillin
- - Length:
  - 3316 bp
- - Type:
  - Mammalian Expression Vectors
- - Replication origin:
  - ori
- - Source/Author:
  - Clontech
- - Copy Number:
  - High copy number
- - Promoter:
  - tight TRE

pmRi-mCherry vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pmRi-mCherry vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pmRi-mCherry.        3316 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-controlled co-expression of a microRNA with 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    pmRi-mCherry.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone the microRNA precursor into the MCS dowstream of the mCherry 
            coding sequence.
FEATURES             Location/Qualifiers
     source          1..3316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1042
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1055..1122
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1230..1311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1493..2081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2255..3112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3113..3217)
                     /label=AmpR promoter

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