pmRi-mCherry vector (V011099)

LOCUS       pmRi-mCherry.        3316 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-controlled co-expression of a microRNA with 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    pmRi-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone the microRNA precursor into the MCS dowstream of the mCherry 
            coding sequence.
FEATURES             Location/Qualifiers
     source          1..3316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1042
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1055..1122
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1230..1311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1493..2081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2255..3112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3113..3217)
                     /label=AmpR promoter