LOCUS       pmRi-mCherry.        3316 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-controlled co-expression of a microRNA with 
            mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    pmRi-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3316)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone the microRNA precursor into the MCS dowstream of the mCherry 
            coding sequence.
FEATURES             Location/Qualifiers
     source          1..3316
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1042
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1055..1122
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1230..1311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1493..2081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2255..3112)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3113..3217)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattcgc caccatggtg agcaagggcg aggaggataa
      361 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      421 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      481 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      541 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      601 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      661 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      721 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      781 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      841 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      901 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      961 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     1021 cggcatggac gagctgtaca agtccggaaa ctagggatcc tctagtcagc tgacgcgtgc
     1081 tagcgcggcc gcatcgataa gcttgtcgac gatatctcta gaggatcata atcagccata
     1141 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1201 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     1261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cctcgagctt
     1321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     1381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     1441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     1501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     1561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     1621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     1681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     2041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     2101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     2161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     2221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     2281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     2341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     2401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     2461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     2521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     2581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     2641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     2701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata
     2881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     3001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     3061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     3121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     3181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     3241 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     3301 ggccctttcg tcttca
//
