pmRi-ZsGreen1 vector (V011098)

Basic Vector Information

      • Vector Name:
      • pmRi-ZsGreen1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3301 bp
      • Type:
      • Mammalian Expression Vectors
      • Replication origin:
      • ori
      • Source/Author:
      • Clontech
      • Copy Number:
      • High copy number
      • Promoter:
      • tight TRE

pmRi-ZsGreen1 vector Vector Map

pmRi-ZsGreen13301 bp6001200180024003000tight TRE promoterZsGreen1MCSSV40 poly(A) signaloriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pmRi-ZsGreen1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pmRi-ZsGreen1.        3301 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-controlled co-expression of a microRNA with 
            ZsGreen1.
ACCESSION   .
VERSION     .
KEYWORDS    pmRi-ZsGreen1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone the microRNA precursor into the MCS dowstream of the ZsGreen1 
            coding sequence.
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1027
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     misc_feature    1040..1107
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1215..1296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2240..3097)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3098..3202)
                     /label=AmpR promoter

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