Basic Vector Information
- Vector Name:
- pmRi-ZsGreen1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3301 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- tight TRE
pmRi-ZsGreen1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pmRi-ZsGreen1 vector Sequence
LOCUS pmRi-ZsGreen1. 3301 bp DNA circular SYN 01-JAN-1980 DEFINITION Vector for doxycycline-controlled co-expression of a microRNA with ZsGreen1. ACCESSION . VERSION . KEYWORDS pmRi-ZsGreen1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3301) AUTHORS Clontech TITLE Direct Submission REFERENCE 2 (bases 1 to 3301) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" COMMENT Clone the microRNA precursor into the MCS dowstream of the ZsGreen1 coding sequence. FEATURES Location/Qualifiers source 1..3301 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 4..318 /label=tight TRE promoter /note="Tet-responsive promoter PTight, consisting of seven tet operator sequences followed by the minimal CMV promoter" CDS 335..1027 /label=ZsGreen1 /note="Zoanthus green fluorescent protein" misc_feature 1040..1107 /label=MCS /note="MCS" /note="multiple cloning site" polyA_signal 1215..1296 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin complement(1478..2066) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2240..3097) /label=AmpR /note="beta-lactamase" promoter complement(3098..3202) /label=AmpR promoter
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