LOCUS       pmRi-ZsGreen1.        3301 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for doxycycline-controlled co-expression of a microRNA with 
            ZsGreen1.
ACCESSION   .
VERSION     .
KEYWORDS    pmRi-ZsGreen1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone the microRNA precursor into the MCS dowstream of the ZsGreen1 
            coding sequence.
FEATURES             Location/Qualifiers
     source          1..3301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1027
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     misc_feature    1040..1107
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1215..1296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1478..2066)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2240..3097)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3098..3202)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattcgc caccatggcc cagtccaagc acggcctgac
      361 caaggagatg accatgaagt accgcatgga gggctgcgtg gacggccaca agttcgtgat
      421 caccggcgag ggcatcggct accccttcaa gggcaagcag gccatcaacc tgtgcgtggt
      481 ggagggcggc cccttgccct tcgccgagga catcttgtcc gccgccttca tgtacggcaa
      541 ccgcgtgttc accgagtacc cccaggacat cgtcgactac ttcaagaact cctgccccgc
      601 cggctacacc tgggaccgct ccttcctgtt cgaggacggc gccgtgtgca tctgcaacgc
      661 cgacatcacc gtgagcgtgg aggagaactg catgtaccac gagtccaagt tctacggcgt
      721 gaacttcccc gccgacggcc ccgtgatgaa gaagatgacc gacaactggg agccctcctg
      781 cgagaagatc atccccgtgc ccaagcaggg catcttgaag ggcgacgtga gcatgtacct
      841 gctgctgaag gacggtggcc gcttgcgctg ccagttcgac accgtgtaca aggccaagtc
      901 cgtgccccgc aagatgcccg actggcactt catccagcac aagctgaccc gcgaggaccg
      961 cagcgacgcc aagaaccaga agtggcacct gaccgagcac gccatcgcct ccggctccgc
     1021 cttgccctcc ggaaactagg gatcctctag tcagctgacg cgtgctagcg cggccgcatc
     1081 gataagcttg tcgacgatat ctctagagga tcataatcag ccataccaca tttgtagagg
     1141 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1201 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1261 tcacaaattt cacaaataaa gcattttttt cactgcctcg agcttcctcg ctcactgact
     1321 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1381 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1441 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1501 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1561 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1621 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1681 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1741 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1801 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1861 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1921 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1981 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2041 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2101 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2161 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2221 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2281 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2341 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2401 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2461 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2521 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2581 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2641 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2701 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2761 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2821 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
     2881 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     2941 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3001 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3061 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3121 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3181 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     3241 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     3301 a
//
