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pTK-neo vector (V011066)

Basic Vector Information

Vector Name:
pTK-neo
Antibiotic Resistance:
Kanamycin
Length:
2872 bp
Type:
Mammalian Expression Vectors
Replication origin:
ori
Source/Author:
Novagen (EMD Millipore)
Selection Marker:
Neomycin/G418(Geneticin)
Copy Number:
High copy number
Promoter:
HSV TK

pTK-neo vector Map

Copy Sequence View Map
pTK-neo2872 bp600120018002400HSV TK promoterNeoR/KanRHSV TK poly(A) signaloriHSV TK promoter

pTK-neo vector Sequence

LOCUS       40924_43508        2872 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a neomycin resistance gene, to select for 
            stable integration of a cotransfected expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        604..749
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             816..1607
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1842..1889
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2218..2806
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2870..2872
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"

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