LOCUS       40924_43508        2872 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding a neomycin resistance gene, to select for 
            stable integration of a cotransfected expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        604..749
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             816..1607
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    1842..1889
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      2218..2806
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2870..2872
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
ORIGIN
        1 gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct gacgcggggg
       61 gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt cttggtggcc
      121 acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc cgtggaggcg
      181 ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc gcgcgcccca
      241 gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg gcggcgacgg
      301 gctcgctggg tcctaggctc cacggggacc gtatacgtgg acaggctctg gagcatccgc
      361 acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac gcggctgacg
      421 cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat cttgtcaccc
      481 ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat ccccgtggcc
      541 cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt ctttagttct
      601 atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac gcagatgcag
      661 tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt ggcctcgaac
      721 accgagcgac cctgcagcga cccgcttaaa ggcctttcaa atatgtatcc gctcatgaga
      781 caatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg
      841 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
      901 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg
      961 tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt
     1021 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1081 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1141 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     1201 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     1261 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg
     1321 aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacgcatg
     1381 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1441 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1501 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1561 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct
     1621 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     1681 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     1741 cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga
     1801 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     1861 taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca
     1921 ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc
     1981 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc
     2041 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt
     2101 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     2161 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     2221 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     2281 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     2341 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     2401 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     2461 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     2521 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     2581 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2641 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2701 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2761 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2821 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgccaa at
//
