Basic Vector Information
- Vector Name:
- pTRE-Dual2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 3884 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Copy Number:
- High copy number
- Promoter:
- tight TRE
pTRE-Dual2 vector Map
pTRE-Dual2 vector Sequence
LOCUS pTRE-Dual2. 3884 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for co-expressing mCherry and another gene with the Tet-On(R)
Advanced or Tet-Off(R) Advanced system.
ACCESSION .
VERSION .
KEYWORDS pTRE-Dual2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3884)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 3884)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..3884
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 4..318
/label=tight TRE promoter
/note="Tet-responsive promoter PTight, consisting of seven
tet operator sequences followed by the minimal CMV
promoter"
CDS 335..1042
/label=mCherry
/note="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
misc_feature 1053..1639
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 1640..1690
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 1798..1879
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2061..2649)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2823..3680)
/label=AmpR
/note="beta-lactamase"
promoter complement(3681..3785)
/label=AmpR promoter
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