LOCUS       pTRE-Dual2.        3884 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for co-expressing mCherry and another gene with the Tet-On(R)
            Advanced or Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Dual2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3884
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     CDS             335..1042
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    1053..1639
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1640..1690
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1798..1879
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2061..2649)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2823..3680)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3681..3785)
                     /label=AmpR promoter
ORIGIN
        1 ctcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
       61 tagagaacga tgtcgagttt actccctatc agtgatagag aacgtatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttat ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      241 gagaacgtat gtcgaggtag gcgtgtacgg tgggaggcct atataagcag agctcgttta
      301 gtgaaccgtc agatcgcctg gagaattcgc caccatggtg agcaagggcg aggaggataa
      361 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      421 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      481 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      541 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      601 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      661 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      721 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      781 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      841 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      901 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      961 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     1021 cggcatggac gagctgtaca agtaggctag cgcccctctc cctccccccc ccctaacgtt
     1081 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     1141 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     1201 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     1261 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     1321 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     1381 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     1441 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     1501 cattgtatgg gatctgatct ggggcctcgg tacacatgct ttacatgtgt ttagtcgagg
     1561 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     1621 tgataatatg gccacaaccg cggccgctag atctggatcc atcgatgtcg acgatatcca
     1681 tatgtctaga ggatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     1741 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     1801 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     1861 aaagcatttt tttcactgcc tcgagcttcc tcgctcactg actcgctgcg ctcggtcgtt
     1921 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     1981 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     2041 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     2101 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     2161 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     2221 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     2281 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     2341 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     2401 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     2461 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     2521 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     2581 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     2641 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     2701 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     2761 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     2821 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     2881 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     2941 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     3001 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     3061 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     3121 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     3181 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     3241 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     3301 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     3361 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     3421 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     3481 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     3541 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     3601 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     3661 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     3721 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     3781 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     3841 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttca
//
