pTRE2 vector (V011055)

LOCUS       V011055                 3770 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011055
VERSION     V011055
KEYWORDS    pTRE2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Resnitzky D, Gossen M, Bujard H, Reed SI.
  TITLE     Acceleration of the G1/S phase transition by expression of cyclins
            D1 and E with an inducible system.
  JOURNAL   Mol. Cell. Biol. 1994;14:1669-79.
   PUBMED   8114703
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3770)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell.
            Biol."; date: "1994"; volume: "14"; pages: "1669-79"
            SGRef: number: 2; type: "Journal Article"
            Also known as pUHD10-3.
FEATURES             Location/Qualifiers
     source          1..3770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    440..538
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     intron          554..1126
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1130..1252
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    1323..1378
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2709..3566)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3567..3671)
                     /label="AmpR promoter"