LOCUS       V011055                 3770 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011055
VERSION     V011055
KEYWORDS    pTRE2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Resnitzky D, Gossen M, Bujard H, Reed SI.
  TITLE     Acceleration of the G1/S phase transition by expression of cyclins
            D1 and E with an inducible system.
  JOURNAL   Mol. Cell. Biol. 1994;14:1669-79.
   PUBMED   8114703
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3770)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell.
            Biol."; date: "1994"; volume: "14"; pages: "1669-79"
            SGRef: number: 2; type: "Journal Article"
            Also known as pUHD10-3.
FEATURES             Location/Qualifiers
     source          1..3770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    15..285
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     promoter        318..356
                     /label="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    440..538
                     /label="MCS"
                     /note="MCS"
                     /note="multiple cloning site"
     intron          554..1126
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             1130..1252
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    1323..1378
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     rep_origin      complement(1947..2535)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2709..3566)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(3567..3671)
                     /label="AmpR promoter"
ORIGIN
        1 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
       61 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
      121 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
      181 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
      241 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
      301 ctcggtaccc gggtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt
      361 tagtgaaccg tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac
      421 accgggaccg atccagcctc cgcggccccg aattcgagct cggtacccgg ggatcctcta
      481 gtcagctgac gcgtgctagc gcggccgcat cgataagctt gtcgacgata tctctagagc
      541 tgagaacttc agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa
      601 attcatgtta tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc
      661 cttgtatcac catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca
      721 accattgtct cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt
      781 gcatttgtaa cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct
      841 ctaatcactt ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta
      901 gagaacaatt gttataatta aatgataagg tagaatattt ctgcatataa attctggctg
      961 gcgtggaaat attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc
     1021 tttatggtta caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc
     1081 gggcccctct gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt
     1141 gctggttgtt gtgctgtctc atcattttgg caaagaattc actcctcagg tgcaggctgc
     1201 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     1261 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     1321 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     1381 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     1441 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     1501 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     1561 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     1621 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     1681 tgtccctctt ctcttatgga gatccctcga ctgcattaat gaatcggcca acgcgcgggg
     1741 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     1801 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     1861 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1921 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1981 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     2041 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     2101 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     2161 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     2221 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     2281 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2341 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2401 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2461 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2521 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2581 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2641 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     2701 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     2761 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     2821 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     2881 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     2941 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     3001 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     3061 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     3121 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     3181 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     3241 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     3301 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     3361 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     3421 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     3481 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     3541 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     3601 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     3661 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     3721 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
//
