LOCUS       Exported                3712 bp DNA     circular SYN 16-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   11111111
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 16, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3712
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(468..3712,1..467)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        99..117
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             179..244
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     CDS             311..328
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      395..442
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      479..934
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        960..1064
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1065..1925
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2096..2684
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2870..3009
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3111..3302)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc
       61 aggacccaac gctgcccgag atctcgatcc cgcgaaatta atacgactca ctatagggag
      121 accacaacgg tttccctcta gaaataattt tgtttaactt taagaaggag atatacatat
      181 gaaatacctg ctgccgaccg ctgctgctgg tctgctgctc ctcgctgccc agccggcgat
      241 ggccatggat atcggaatta attcggatcc gaattcgagc tccgtcgaca agcttgcggc
      301 cgcactcgag caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga
      361 agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
      421 acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac
      481 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      541 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      601 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      661 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      721 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      781 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      841 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      901 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
      961 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     1021 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     1081 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     1141 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     1201 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     1261 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     1321 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     1381 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     1441 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1501 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1561 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     1621 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1681 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1741 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1801 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1861 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1921 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1981 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     2041 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2101 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2161 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2221 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2281 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     2341 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     2401 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     2461 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2521 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2581 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2641 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2701 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2761 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2821 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2881 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2941 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     3001 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     3061 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     3121 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     3181 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     3241 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     3301 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     3361 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     3421 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     3481 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     3541 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3601 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3661 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gc
//
