LOCUS       Exported                3662 bp ds-DNA     circular SYN 13-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 13, 2025 from SnapGene 4.1.9
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          join(3422..3662,1..3421)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        96..114
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             176..208
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             258..275
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      342..389
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      426..881
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        907..1011
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1012..1872
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2043..2631
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    2817..2956
                     /label=bom
                     /note="basis of mobility region from pBR322"
     CDS             complement(3058..3249)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg
       61 acccaacgct gcccgagatc tcgatcccgc gaaattaata cgactcacta tagggagacc
      121 acaacggttt ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatggc
      181 tagcatgact ggtggacagc aaatgggtcg cggatccgaa ttcgagctcc gtcgacaagc
      241 ttgcggccgc actcgagcac caccaccacc accactgaga tccggctgct aacaaagccc
      301 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
      361 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
      421 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      481 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      541 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      601 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      661 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      721 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      781 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      841 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
      901 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      961 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1021 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1081 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1141 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1201 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1261 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1321 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     1381 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     1441 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     1501 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca
     1561 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     1621 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     1681 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     1741 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     1801 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1861 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1921 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1981 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2041 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2101 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2161 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2221 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2281 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2341 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2401 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     2461 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2521 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2581 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     2641 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     2701 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     2761 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     2821 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     2881 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     2941 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3001 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3061 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     3121 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     3181 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     3241 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     3301 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     3361 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     3421 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     3481 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     3541 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     3601 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     3661 ac
//
