Basic Vector Information
pVP16 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVP16 vector Sequence
LOCUS 40924_46053 3280 bp DNA circular SYN 18-DEC-2018
DEFINITION Cloning vector pVP16, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3280)
AUTHORS Holtz A, Lou Y.
TITLE pVP16 complete sequence
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 3280)
AUTHORS Holtz A, Lou Y.
TITLE Direct Submission
JOURNAL Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East
Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE 3 (bases 1 to 3280)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3280)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle,
Palo Alto, CA 94303-4230, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT This vector can be obtained from CLONTECH Laboratories, Inc., 1020
East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
call (415) 424-8222 or (800) 662-2566, extension 1. International
customers, please contact your local distributor. For technical
information, call (415) 424-8222 or (800) 662-2566, extension 3.
This sequence has been compiled from information in the sequence
databases, published literature and other sources, together with
partial sequences obtained by CLONTECH. If you suspect there is an
error in this sequence, please contact CLONTECH's Technical Support
Department at (415) 424-8222 or (800) 662-2566, extension 3 or
E-mail TECH@CLONTECH.COM.
FEATURES Location/Qualifiers
source 1..3280
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 20..38
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
promoter 67..364
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
regulatory 385..394
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 397..417
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 418..651
/label=VP16 AD
/note="transcriptional activation domain of herpes simplex
virus protein VP16 (Triezenberg et al., 1988; Cousens et
al., 1989)"
polyA_signal 809..943
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(954..972)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(1230..1818)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1992..2849)
/label=AmpR
/note="beta-lactamase"
promoter complement(2850..2954)
/label=AmpR promoter
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