LOCUS       40924_46053        3280 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pVP16, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Holtz A, Lou Y.
  TITLE     pVP16 complete sequence
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3280)
  AUTHORS   Holtz A, Lou Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303-4230, USA
REFERENCE   3  (bases 1 to 3280)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3280)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-FEB-1997) CLONTECH Laboratories, Inc., 1020 East Meadow Circle, 
            Palo Alto, CA 94303-4230, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     This vector can be obtained from CLONTECH Laboratories, Inc., 1020 
            East Meadow Circle, Palo Alto, CA 94303-4230, USA. To place an order
            call (415) 424-8222 or (800) 662-2566, extension 1. International 
            customers, please contact your local distributor. For technical 
            information, call (415) 424-8222 or (800) 662-2566, extension 3. 
            This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. If you suspect there is an 
            error in this sequence, please contact CLONTECH's Technical Support 
            Department at (415) 424-8222 or (800) 662-2566, extension 3 or 
            E-mail TECH@CLONTECH.COM.
FEATURES             Location/Qualifiers
     source          1..3280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        67..364
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     regulatory      385..394
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             397..417
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             418..651
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     polyA_signal    809..943
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(954..972)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1230..1818)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1992..2849)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2850..2954)
                     /label=AmpR promoter
ORIGIN
        1 tatgtatcat acacatacga tttaggtgac actatagaac tcgactgtgg aatgtgtgtc
       61 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
      121 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
      181 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
      241 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
      301 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga agaggctttt
      361 ttggaggaga tctaagctag cgccgccacc atgggcccta aaaagaagcg taaagtcgcc
      421 cccccgaccg atgtcagcct gggggacgag ctccacttag acggcgagga cgtggcgatg
      481 gcgcatgccg acgcgctaga cgatttcgat ctggacatgt tgggggacgg ggattccccg
      541 gggccgggat ttacccccca cgactccgcc ccctacggcg ctctggatat ggccgacttc
      601 gagtttgagc agatgtttac cgatgccctt ggaattgacg agtacggtgg ggaattcccg
      661 gggatccgtc gacgcgtctg cagaagcttc tagataagta atgatcataa tcagccatac
      721 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      781 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      841 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      901 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgccggt ctccctatag
      961 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
     1021 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
     1081 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     1141 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1201 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1261 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1321 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1381 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1441 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1501 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1561 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1621 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1681 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1741 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1801 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1861 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1921 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1981 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     2041 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     2101 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     2161 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2221 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2281 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt gacgctcgtc gtttggtatg
     2341 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2401 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2461 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2521 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2581 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2641 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2701 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2761 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2821 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2881 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2941 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     3001 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     3061 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     3121 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3181 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac
     3241 atattgtcgt tagaacgcgg ctacaattaa tacataacct
//
