pLVX-Puro vector (V010426)

Price Information

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V010426 pLVX-Puro In stock, instant shipping

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Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

The pLVX-Puro is a lentiviral expression vector with the constitutive CMV promoter and a puromycin resistance marker.

Vector Name:
Antibiotic Resistance:
8102 bp
Viral Expression & Packaging Vectors
Replication origin:
Copy Number:
High copy number

pLVX-Puro vector Vector Map

pLVX-Puro8102 bp4008001200160020002400280032003600400044004800520056006000640068007200760080003' LTRHIV-1 PsiRREgp41 peptideProtein TatcPPT/CTSCMV enhancerCMV promoterMCSPGK promoterPuroRWPRE5' LTRM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterSV40 poly(A) signal

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pLVX-Puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V010426                 8102 bp    DNA     circular SYN 01-JAN-1980
VERSION     V010426
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8102)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2027..2144
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
     misc_feature    2802..2867
                     /note="multiple cloning site"
     promoter        2890..3389
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3410..4006
                     /note="puromycin N-acetyltransferase"
     misc_feature    4023..4611
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4818..5451
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5580..5596)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
     protein_bind    complement(5604..5620)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5628..5658)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5673..5694)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5982..6570)
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
     CDS             complement(6744..7601)
     promoter        complement(7602..7706)
                     /label="AmpR promoter"
     polyA_signal    7754..7888
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"