LOCUS       Exported                8055 bp DNA     circular SYN 10-SEP-2025
DEFINITION  Exported.
ACCESSION   V010426
VERSION     .
KEYWORDS    pLVX-Puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8055)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8055)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 10, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          join(6799..8055,1..6798)
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    46..279
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             464..508
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             657..698
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    770..887
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        944..1247
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1248..1451
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    1545..1610
                     /label=MCS
                     /note="multiple cloning site"
     promoter        1633..2132
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2153..2749
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    2766..3354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3561..4194
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4323..4339)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4347..4363)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4371..4401)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4416..4437)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4725..5313)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5487..6344)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6345..6449)
                     /label=AmpR promoter
     polyA_signal    6497..6631
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             6799..7432
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7479..7604
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
ORIGIN
        1 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
       61 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
      121 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
      181 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
      241 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
      301 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
      361 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
      421 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
      481 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
      541 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
      601 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
      661 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
      721 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc tttaaaagaa
      781 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      841 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      901 gggacagcag agatccagtt tatcgataag cttgggagtt ccgcgttaca taacttacgg
      961 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
     1021 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
     1081 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
     1141 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
     1201 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
     1261 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
     1321 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
     1381 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
     1441 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
     1501 acctccatag aagacaccga ctctactaga ggatcgctag cgctaccgga ctcagatctc
     1561 gagctcaagc ttcgaattct gcagtcgacg gtaccgcggg cccgggatcc cgcgactcta
     1621 gataattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     1681 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     1741 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     1801 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     1861 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     1921 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     1981 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     2041 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     2101 tgttctcctc ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatgaccga
     2161 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     2221 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     2281 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     2341 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     2401 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     2461 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     2521 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     2581 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     2641 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     2701 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaccgcgtct
     2761 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     2821 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     2881 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     2941 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3001 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3061 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3121 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3181 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     3241 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     3301 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     3361 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     3421 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     3481 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3541 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     3601 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     3661 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     3721 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     3781 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     3841 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     3901 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     3961 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     4021 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4081 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4141 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     4201 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     4261 agaggccttg acattgctag cgttttaccg tcgacctcta gctagagctt ggcgtaatca
     4321 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4381 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4441 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4501 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4561 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4621 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4681 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4741 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4801 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4861 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4921 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4981 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5041 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5101 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5161 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5221 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5281 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5341 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5401 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5461 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5521 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5581 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5641 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5701 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5761 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5821 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5881 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5941 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6001 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6061 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6121 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6181 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6241 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6301 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6361 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6421 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     6481 gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6541 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6601 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
     6661 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
     6721 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
     6781 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
     6841 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
     6901 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
     6961 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
     7021 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
     7081 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
     7141 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
     7201 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
     7261 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     7321 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     7381 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     7441 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     7501 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     7561 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     7621 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     7681 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     7741 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     7801 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     7861 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     7921 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     7981 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8041 agtagcaccc accaa
//
