LOCUS       Exported                6530 bp DNA     circular SYN 02-SEP-2024
DEFINITION  Cloning vector pESC-TRP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Lu Q.
  TITLE     pESC-TRP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   Lu Q.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-1998) Technical Services, Stratagene, 11011 N. 
            Torrey Pines Rd., La Jolla, CA 92037, USA
REFERENCE   3  (bases 1 to 6530)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6530)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 6530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-MAY-1998) Technical Services, Stratagene, 11011 N. Torrey Pines
            Rd., La Jolla, CA 92037, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     SGRef: number: 4; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        191..472
                     /label=TRP1 promoter
     CDS             473..1144
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     rep_origin      complement(1250..1705)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     terminator      complement(1784..1949)
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             complement(2097..2120)
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     promoter        2167..2831
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     promoter        2842..2860
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             2878..2907
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     terminator      2937..3126
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3369..3957)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4131..4988)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4989..5093)
                     /label=AmpR promoter
     rep_origin      complement(5120..6462)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatagatc aacgacatta ctatatatat aatataggaa gcatttaata gaacagcatc
      241 gtaatatatg tgtactttgc agttatgacg ccagatggca gtagtggaag atattcttta
      301 ttgaaaaata gcttgtcacc ttacgtacaa tcttgatccg gagcttttct ttttttgccg
      361 attaagaatt aattcggtcg aaaaaagaaa aggagagggc caagagggag ggcattggtg
      421 actattgagc acgtgagtat acgtgattaa gcacacaaag gcagcttgga gtatgtctgt
      481 tattaatttc acaggtagtt ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga
      541 ggccgcagaa tgtgctctag attccgatgc tgacttgctg ggtattatat gtgtgcccaa
      601 tagaaagaga acaattgacc cggttattgc aaggaaaatt tcaagtcttg taaaagcata
      661 taaaaatagt tcaggcactc cgaaatactt ggttggcgtg tttcgtaatc aacctaagga
      721 ggatgttttg gctctggtca atgattacgg cattgatatc gtccaactgc atggagatga
      781 gtcgtggcaa gaataccaag agttcctcgg tttgccagtt attaaaagac tcgtatttcc
      841 aaaagactgc aacatactac tcagtgcagc ttcacagaaa cctcattcgt ttattccctt
      901 gtttgattca gaagcaggtg ggacaggtga acttttggat tggaactcga tttctgactg
      961 ggttggaagg caagagagcc ccgaaagctt acattttatg ttagctggtg gactgacgcc
     1021 agaaaatgtt ggtgatgcgc ttagattaaa tggcgttatt ggtgttgatg taagcggagg
     1081 tgtggagaca aatggtgtaa aagactctaa caaaatagca aatttcgtca aaaatgctaa
     1141 gaaataggtt attactgagt agtatttatt taagtattgt ttgtgcactt gccgatctat
     1201 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga aattgtaagc
     1261 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
     1321 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
     1381 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
     1441 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
     1501 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
     1561 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
     1621 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
     1681 cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac tgttgggaag
     1741 ggcgatcggt gcgggcctct tcgctattac gccagctgaa ttggagcgac ctcatgctat
     1801 acctgagaaa gcaacctgac ctacaggaaa gagttactca agaataagaa ttttcgtttt
     1861 aaaacctaag agtcacttta aaatttgtat acacttattt tttttataac ttatttaata
     1921 ataaaaatca taaatcataa gaaattcgct tatttagaag tgtcaacaac gtatctacca
     1981 acgatttgac ccttttccat cttttcgtaa atttctggca aggtagacaa gccgacaacc
     2041 ttgattggag acttgaccaa acctctggcg aagaattgtt aattaagagc tcagatctta
     2101 tcgtcgtcat ccttgtaatc catcgatact agtgcggccg ccctttagtg agggttgaat
     2161 tcgaattttc aaaaattctt actttttttt tggatggacg caaagaagtt taataatcat
     2221 attacatggc attaccacca tatacatatc catatacata tccatatcta atcttactta
     2281 tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt ctctttggaa
     2341 ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag aagccgccga
     2401 gcgggtgaca gccctccgaa ggaagactct cctccgtgcg tcctcgtctt caccggtcgc
     2461 gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat
     2521 actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca
     2581 aatgaacgaa tcaaattaac aaccatagga tgataatgcg attagttttt tagccttatt
     2641 tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca
     2701 aaaactgcat aaccacttta actaatactt tcaacatttt cggtttgtat tacttcttat
     2761 tcaaatgtaa taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa
     2821 ggagaaaaaa ccccggatcc gtaatacgac tcactatagg gcccgggcgt cgacatggaa
     2881 cagaagttga tttccgaaga agacctcgag taagcttggt accgcggcta gctaagatcc
     2941 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     3001 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     3061 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     3121 tcgaagatcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     3181 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     3241 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     3301 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3361 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3421 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3481 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3541 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3601 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3661 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3721 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3781 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3841 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3901 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3961 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     4021 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     4081 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     4141 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     4201 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     4261 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     4321 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4381 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4441 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4501 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4561 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4621 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4681 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4741 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4801 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4861 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4921 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4981 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     5041 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     5101 ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa
     5161 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
     5221 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa
     5281 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag
     5341 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac
     5401 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc
     5461 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt
     5521 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc
     5581 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc
     5641 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg
     5701 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta
     5761 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac
     5821 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag
     5881 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca
     5941 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt
     6001 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc
     6061 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa
     6121 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca
     6181 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca
     6241 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat
     6301 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg
     6361 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt
     6421 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat
     6481 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
//
