pFA6a vector (V010285)

LOCUS       40924_19661        2512 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Precursor to plasmids carrying kanMX and other selector modules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Wach A, Brachat A, Pohlmann R, Philippsen P.
  TITLE     New heterologous modules for classical or PCR-based gene disruptions
            in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1994;10:1793-808.
  PUBMED    7747518
REFERENCE   2  (bases 1 to 2512)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2512)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1994"; volume: "10"; pages: "1793-808"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(150..168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(426..1014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1188..2045)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2046..2150)
                     /label=AmpR promoter
     promoter        2496..2512
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"