LOCUS       40924_19661        2512 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Precursor to plasmids carrying kanMX and other selector modules.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Wach A, Brachat A, Pohlmann R, Philippsen P.
  TITLE     New heterologous modules for classical or PCR-based gene disruptions
            in Saccharomyces cerevisiae.
  JOURNAL   Yeast 1994;10:1793-808.
  PUBMED    7747518
REFERENCE   2  (bases 1 to 2512)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2512)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1994"; volume: "10"; pages: "1793-808"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(150..168)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(426..1014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1188..2045)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2046..2150)
                     /label=AmpR promoter
     promoter        2496..2512
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatcccc gggttaatta
       61 aggcgcgcca gatctgttta aacgagctcg aattcatcga tgatatcaga tccactagtg
      121 gcctatgcgg ccgcggatct gccggtctcc ctatagtgag tcgtattaat ttcgataagc
      181 caggttaacc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      601 ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      841 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
      901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2161 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2221 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2281 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2341 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2401 tcagagcaga ttgtactgag agtgcaccat atggacatat tgtcgttaga acgcggctac
     2461 aattaataca taaccttatg tatcatacac atacgattta ggtgacacta ta
//
