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pGAPZ B vector (V010275)

Basic Vector Information

Vector Name:
pGAPZ B
Antibiotic Resistance:
Bleomycin
Length:
2882 bp
Type:
Yeast Plasmids
Replication origin:
ori
Host:
Yeast
Source/Author:
Invitrogen (Life Technologies)
Copy Number:
High copy number
Promoter:
GAP

pGAPZ B vector Map

Copy Sequence View Map
pGAPZ B2882 bp600120018002400GAP promoterMyc6xHisAOX1 terminatorTEF1 promoterEM7 promoterBleoRCYC1 terminatorori

pGAPZ B vector Sequence

LOCUS       40924_21110        2882 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2882)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             562..591
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             607..624
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      703..949
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        964..1375
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1382..1429
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1448..1819
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1888..2135
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2210..2798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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