LOCUS       40924_21110        2882 bp DNA     circular SYN 13-SEP-2021
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2882
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             562..591
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             607..624
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     terminator      703..949
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        964..1375
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1382..1429
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1448..1819
                     /codon_start=1
                     /label=BleoR
                     /note="antibiotic-binding protein"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     terminator      1888..2135
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2210..2798)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
       61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
      121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
      181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
      241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
      301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
      361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
      421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
      481 tatttcgaaa cgaggaattc acgtggccca gccggccgtc tcggatcggt acctcgagcc
      541 gcggcggccg ccagctttct agaacaaaaa ctcatctcag aagaggatct gaatagcgcc
      601 gtcgaccatc atcatcatca tcattgagtt ttagccttag acatgactgt tcctcagttc
      661 aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat
      721 gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata
      781 gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta
      841 tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt
      901 tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt
      961 gcggatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat
     1021 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat
     1081 tttccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa
     1141 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg
     1201 tttctttttc ttgaaatttt tttttttagt ttttttctct ttcagtgacc tccattgata
     1261 tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt
     1321 acaacttttt ttacttcttg ttcattagaa agaaagcata gcaatctaat ctaagggcgg
     1381 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     1441 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     1501 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     1561 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     1621 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     1681 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     1741 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     1801 cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca ggcctcggag
     1861 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac
     1921 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg
     1981 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt
     2041 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg
     2101 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa
     2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     2401 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgaga
     2881 tc
//
