pGBDU-C2 vector (V010266)

LOCUS       pGBDU-C2.        5995 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector with a URA3 marker for fusing a
            gene to the GAL4 DNA binding domain. For other reading frames, use 
            pGBDU-C1 or pGBDU-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGBDU-C2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5995)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5995)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..917
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1292..1479
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1490..1706
                     /label=URA3 promoter
     CDS             1707..2507
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(2825..3413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3587..4444)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4445..4549)
                     /label=AmpR promoter
     rep_origin      4831..5995
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"