LOCUS       pGBDU-C2.        5995 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector with a URA3 marker for fusing a
            gene to the GAL4 DNA binding domain. For other reading frames, use 
            pGBDU-C1 or pGBDU-C3.
ACCESSION   .
VERSION     .
KEYWORDS    pGBDU-C2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5995)
  AUTHORS   James P, Halladay J, Craig EA.
  TITLE     Genomic libraries and a host strain designed for highly efficient 
            two-hybrid selection in yeast.
  JOURNAL   Genetics 1996;144:1425-36.
  PUBMED    8978031
REFERENCE   2  (bases 1 to 5995)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5995)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1996"; volume: "144"; pages: "1425-36"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..406
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             434..874
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    878..917
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     terminator      1292..1479
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        1490..1706
                     /label=URA3 promoter
     CDS             1707..2507
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(2825..3413)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3587..4444)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4445..4549)
                     /label=AmpR promoter
     rep_origin      4831..5995
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
      421 agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
      481 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      541 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      601 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      661 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      721 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      781 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      841 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccatcgatg
      901 tcgacctgca gagatctatg aatcgtagat actgaaaaac cccgcaagtt cacttcaact
      961 gtgcatcgtg caccatctca atttctttca tttatacatc gttttgcctt cttttatgta
     1021 actatactcc tctaagtttc aatcttggcc atgtaacctc tgatctatag aattttttaa
     1081 atgactagaa ttaatgccca tctttttttt ggacctaaat tcttcatgaa aatatattac
     1141 gagggcttat tcagaagctt tggacttctt cgccagaggt ttggtcaagt ctccaatcaa
     1201 ggttgtcggc ttgtctacct tgccagaaat ttacgaaaag atggaaaagg gtcaaatcgt
     1261 tggtagatac gttgttgaca cttctaaata agcgaatttc ttatgattta tgatttttat
     1321 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt
     1381 aaaacgaaaa ttcttgttct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt
     1441 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcagcttt tcaattcaat
     1501 tcatcatttt ttttttattc ttttttttga tttcggtttc tttgaaattt ttttgattcg
     1561 gtaatctccg aacagaagga agaacgaagg aaggagcaca gacttagatt ggtatatata
     1621 cgcatatgta gtgttgaaga aacatgaaat tgcccagtat tcttaaccca actgcacaga
     1681 acaaaaaccg gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta
     1741 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact
     1801 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag
     1861 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg
     1921 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca
     1981 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa
     2041 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg
     2101 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag
     2161 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg
     2221 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag
     2281 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg
     2341 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta
     2401 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca
     2461 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat
     2521 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt
     2581 tattacccct ggattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2641 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2701 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2761 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2821 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2881 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2941 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     3001 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     3061 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3121 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3181 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3241 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
     3301 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3361 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3421 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3481 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3541 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3601 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3661 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3721 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3781 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3841 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3901 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3961 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     4021 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     4081 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4141 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4201 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4261 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4321 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4381 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4441 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4501 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4561 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     4621 ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac
     4681 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
     4741 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat
     4801 cagagcagat tgtactgaga gtgcaccata acgcatttaa gcataaacac gcactatgcc
     4861 gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga cgtgaacagt
     4921 gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt
     4981 gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact tcagagcgct
     5041 tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg caccggactg
     5101 taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa aagtatctct
     5161 ttgctatata tctctgtgct atatccctat ataacctacc catccacctt tcgctccttg
     5221 aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat tactctttag
     5281 acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac gaaaatgtaa
     5341 acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata attttctgac
     5401 caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa tccacatcgg
     5461 tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc ttcgctagta
     5521 atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat agacaccaaa
     5581 gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga gactgcatta
     5641 tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa atagcgctct
     5701 cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta aaatagcgct
     5761 ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg aaaaattagc
     5821 gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt tggtaaaata
     5881 gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt gtttgaaaaa
     5941 ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct tcgtt
//
