Basic Vector Information
- Vector Name:
- pOBD2
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7269 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR,
- Copy Number:
- High copy number
- Promoter:
- ADH1
pOBD2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOBD2 vector Sequence
LOCUS 40924_33712 7269 bp DNA circular SYN 01-JAN-1980
DEFINITION Yeast two-hybrid """"bait"""" vector for fusing a gene to the GAL4
DNA binding domain.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7269)
AUTHORS Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR,
Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li
Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M,
Johnston M, Fields S, Rothberg JM.
TITLE A comprehensive analysis of protein-protein interactions in
Saccharomyces cerevisiae.
JOURNAL Nature 2000;403:623-7.
PUBMED 10688190
REFERENCE 2 (bases 1 to 7269)
AUTHORS Fields Lab
TITLE Direct Submission
REFERENCE 3 (bases 1 to 7269)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nature";
date: "2000"; volume: "403"; pages: "623-7"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7269
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 112..508
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
CDS 536..976
/codon_start=1
/label=GAL4 DNA binding domain
/note="DNA binding domain of the GAL4 transcriptional
activator"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
terminator 1458..1645
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
misc_feature complement(2477..3639)
/label=CEN/ARS
/note="S. cerevisiae CEN4 centromere fused to the
autonomously replicating sequence ARS1/ARS416"
promoter complement(4153..4254)
/label=TRP1 promoter
promoter 4360..4464
/label=AmpR promoter
CDS 4465..5322
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5496..6084
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 7073..7269
/label=ADH1 promoter
/note="promoter for alcohol dehydrogenase 1"
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