LOCUS       40924_33712        7269 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast two-hybrid """"bait"""" vector for fusing a gene to the GAL4 
            DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7269)
  AUTHORS   Uetz P, Giot L, Cagney G, Mansfield TA, Judson RS, Knight JR, 
            Lockshon D, Narayan V, Srinivasan M, Pochart P, Qureshi-Emili A, Li 
            Y, Godwin B, Conover D, Kalbfleisch T, Vijayadamodar G, Yang M, 
            Johnston M, Fields S, Rothberg JM.
  TITLE     A comprehensive analysis of protein-protein interactions in 
            Saccharomyces cerevisiae.
  JOURNAL   Nature 2000;403:623-7.
  PUBMED    10688190
REFERENCE   2  (bases 1 to 7269)
  AUTHORS   Fields Lab
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7269)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2000"; volume: "403"; pages: "623-7"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        112..508
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             536..976
                     /codon_start=1
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     terminator      1458..1645
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     misc_feature    complement(2477..3639)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(4153..4254)
                     /label=TRP1 promoter
     promoter        4360..4464
                     /label=AmpR promoter
     CDS             4465..5322
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5496..6084
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        7073..7269
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
ORIGIN
        1 gtggtacata acgaactaat actgtagccc tagacttgat agccatcatc atatcgaagt
       61 ttcactaccc tttttccatt tgccatctat tgaagtaata ataggcgcat gcaacttctt
      121 ttcttttttt ttcttttctc tctcccccgt tgttgtctca ccatatccgc aatgacaaaa
      181 aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa cagatgtcgt
      241 tgttccagag ctgatgaggg gtatctcgaa gcacacgaaa ctttttcctt ccttcattca
      301 cgcacactac tctctaatga gcaacggtat acggccttcc ttccagttac ttgaatttga
      361 aataaaaaaa agtttgctgt cttgctatca agtataaata gacctgcaat tattaatctt
      421 ttgtttcctc gtcattgttc tcgttccctt tcttccttgt ttctttttct gcacaatatt
      481 tcaagctata ccaagcatac aatcaactcc aagcttgaag caagcctcct gaaagatgaa
      541 gctactgtct tctatcgaac aagcatgcga tatttgccga cttaaaaagc tcaagtgctc
      601 caaagaaaaa ccgaagtgcg ccaagtgtct gaagaacaac tgggagtgtc gctactctcc
      661 caaaaccaaa aggtctccgc tgactagggc acatctgaca gaagtggaat caaggctaga
      721 aagactggaa cagctatttc tactgatttt tcctcgagaa gaccttgaca tgattttgaa
      781 aatggattct ttacaggata taaaagcatt gttaacagga ttatttgtac aagataatgt
      841 gaataaagat gccgtcacag atagattggc ttcagtggag actgatatgc ctctaacatt
      901 gagacagcat agaataagtg cgacatcatc atcggaagag agtagtaaca aaggtcaaag
      961 acagttgact gtatcgaact atctattcga tgatgaagat accccaccaa acccaaaaaa
     1021 agagatcgaa ttccagctga ccaccatggc aattcccggg gatccgtcga cctgcagaga
     1081 tctatgaatc gtagatactg aaaaaccccg caagttcact tcaactgtgc atcgtgcacc
     1141 atctcaattt ctttcattta tacatcgttt tgccttcttt tatgtaacta tactcctcta
     1201 agtttcaatc ttggccatgt aacctctgat ctatagaatt ttttaaatga ctagaattaa
     1261 tgcccatctt ttttttggac ctaaattctt catgaaaata tattacgagg gcttattcag
     1321 aagctttgga cttcttcgcc agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt
     1381 ctaccttgcc agaaatttac gaaaagatgg aaaagggtca aatcgttggt agatacgttg
     1441 ttgacacttc taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta
     1501 taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct
     1561 tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg
     1621 ctcttattga ccacacctct accggcatgc cgagcaaatg cctgcaaatc gctccccatt
     1681 tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg tgtgtatttt
     1741 atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcct ggcgtaatag
     1801 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     1861 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatcgctg
     1921 ggccattctc atgaagaata tcttgaattt attgtcatat tactagttgg tgtggaagtc
     1981 catatatcgg tgatcaatat agtggttgac atgctggcta gtcaacattg agccttttga
     2041 tcatgcaaat atattacggt attttacaat caaatatcaa acttaactat tgactttata
     2101 acttatttag gtggtaacat tcttataaaa aagaaaaaaa ttactgcaaa acagtactag
     2161 cttttaactt gtatcctagg ttatctatgc tgtctcacca tagagaatat tacctatttc
     2221 agaatgtatg tccatgattc gccgggtaaa tacatataat acacaaatct ggcttaataa
     2281 agtctataat atatctcata aagaagtgct aaattggcta gtgctatata tttttaagaa
     2341 aatttctttt gactaagtcc atatcgactt tgtaaaagtt cactttagca tacatatatt
     2401 acacgagcca gaaattgtaa cttttgccta aaatcacaaa ttgcaaaatt taattgcttg
     2461 caaaaggtca catgcttata atcaactttt ttaaaaattt aaaatacttt tttatttttt
     2521 atttttaaac ataaatgaaa taatttattt attgtttatg attaccgaaa cataaaacct
     2581 gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc actacctagg agcggccaaa
     2641 atgccgaggc tttcatagct taaactcttt acagaaaata ggcattatag atcagttcga
     2701 gttttcttat tcttccttcc ggttttatcg tcacagtttt acagtaaata agtatcacct
     2761 cttagagttc gatgataagc tgtcaaacat gagaattaat tccacatgtt aaaatagtga
     2821 aggagcatgt tcggcacaca gtggaccgaa cgtggggtaa gtgcactagg gtccggttaa
     2881 acggatctcg cattgatgag gcaacgctaa ttatcaacat atagattgtt atctatctgc
     2941 atgaacacga aatctttact tgacgacttg aggctgatgg tgtttatgca aagaaaccac
     3001 tgtgtttaat atgtgtcact gtttgatatt actgtcagcg tagaagataa tagtaaaagc
     3061 ggttaataag tgtatttgag ataagtgtga taaagttttt acagcgaaaa gacgataaat
     3121 acaagaaaat gattacgagg atacggagag aggtatgtac atgtgtattt atatactaag
     3181 ctgccggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc attgtagcgt
     3241 atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa tggtaaaagt
     3301 caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat gatttagcat
     3361 tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc ttgcttttca
     3421 aaaggcttgc aggcaagtgc acaaacaata cttaaataaa tactactcag taataaccta
     3481 tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac catttgtctc
     3541 cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat caccaacatt
     3601 ttctggcgtc agtccaccag ctaacataaa atgtaagctc tcggggctct cttgccttcc
     3661 aacccagtca gaaatcgagt tccaatccaa aagttcacct gtcccacctg cttctgaatc
     3721 aaacaaggga ataaacgaat gaggtttctg tgaagctgca ctgagtagta tgttgcagtc
     3781 ttttggaaat acgagtcttt taataactgg caaaccgagg aactcttggt attcttgcca
     3841 cgactcatct ccatgcagtt ggacgatatc aatgccgtaa tcattgacca gagccaaaac
     3901 atcctcctta ggttgattac gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt
     3961 tttatatgct tttacaagac ttgaaatttt ccttgcaata accgggtcaa ttgttctctt
     4021 tctattgggc acacatataa tacccagcaa gtcagcatcg gaatctagtg cacattctgc
     4081 ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac ctgtgaaatt
     4141 aataacagac atactccaag ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa
     4201 tagtcaccaa tgccctccct cttggccctc tccttttctt ttttcgaccg aattaattct
     4261 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4321 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4381 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4441 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4501 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4561 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4621 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4681 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     4741 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4801 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4861 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     4921 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     4981 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5041 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5101 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5161 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5221 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5281 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5341 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5401 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5461 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5521 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5581 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5641 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5701 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5761 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5821 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     5881 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     5941 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6001 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6061 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6121 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6181 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6241 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6301 tggccgattc attaatgcag gatccgggat cgaagaaatg atggtaaatg aaataggaaa
     6361 tcaaggagca tgaaggcaaa agacaaatat aagggtcgaa cgaaaaataa agtgaaaagt
     6421 gttgatatga tgtatttggc tttgcggcgc cgaaaaaacg agtttacgca attgcacaat
     6481 catgctgact ctgtggcgga cccgcgctct tgccggcccg gcgataacgc tgggcgtgag
     6541 gctgtgcccg gcggagtttt ttgcgcctgc attttccaag gtttaccctg cgctaagggg
     6601 cgagattgga gaagcaataa gaatgccggt tggggttgcg atgatgacga ccacgacaac
     6661 tggtgtcatt atttaagttg ccgaaagaac ctgagtgcat ttgcaacatg agtatactag
     6721 aagaatgagc caagacttgc gagacgcgag tttgccggtg gtgcgaacaa tagagcgacc
     6781 atgaccttga aggtgagacg cgcataaccg ctagagtact ttgaagagga aacagcaata
     6841 gggttgctac cagtataaat agacaggtac atacaacact ggaaatggtt gtctgtttga
     6901 gtacgctttc aattcatttg ggtgtgcact ttattatgtt acaatatgga agggaacttt
     6961 acacttctcc tatgcacata tattaattaa agtccaatgc tagtagagaa ggggggtaac
     7021 acccctccgc gctcttttcc gatttttttc taaaccgtgg aatatttcgg atatcctttt
     7081 gttgtttccg ggtgtacaat atggacttcc tcttttctgg caaccaaacc catacatcgg
     7141 gattcctata ataccttcgt tggtctccct aacatgtagg tggcggaggg gagatataca
     7201 atagaacaga taccagacaa gacataatgg gctaaacaag actacaccaa ttacactgcc
     7261 tcattgatg
//
