Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V010214 | pUCATPH | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pUCATPH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5086 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Source/Author:
- Yang G, Turgeon BG, Yoder OC.
- Copy Number:
- High copy number
- Promoter:
- trpC
- 5' Primer:
- M13 fwd
- 3' Primer:
- M13 rev
pUCATPH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pUCATPH vector Sequence
LOCUS 40924_45243 5086 bp DNA circular SYN 01-JAN-1980
DEFINITION Hygromycin resistance plasmid for restriction enzyme-mediated
integration (REMI) mutagenesis of fungi.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5086)
AUTHORS Yang G, Turgeon BG, Yoder OC.
TITLE Toxin-deficient mutants from a toxin-sensitive transformant of
Cochliobolus heterostrophus.
JOURNAL Genetics 1994;137:751-7.
PUBMED 8088521
REFERENCE 2 (bases 1 to 5086)
TITLE Direct Submission
REFERENCE 3 (bases 1 to 5086)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Genetics";
date: "1994"; volume: "137"; pages: "751-7"
COMMENT SGRef: number: 2; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5086
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 379..395
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator complement(704..1262)
/label=trpC terminator
/note="transcription terminator from the Aspergillus
nidulans trpC gene"
CDS complement(1434..2456)
/codon_start=1
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(2461..2817)
/label=trpC promoter
/note="promoter for Aspergillus nidulans trpC"
primer_bind complement(2865..2881)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(2889..2905)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2913..2943)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(2958..2979)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3267..3855)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4029..4886)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4887..4991)
/label=AmpR promoter