LOCUS       40924_45243        5086 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Hygromycin resistance plasmid for restriction enzyme-mediated 
            integration (REMI) mutagenesis of fungi.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Yang G, Turgeon BG, Yoder OC.
  TITLE     Toxin-deficient mutants from a toxin-sensitive transformant of 
            Cochliobolus heterostrophus.
  JOURNAL   Genetics 1994;137:751-7.
  PUBMED    8088521
REFERENCE   2  (bases 1 to 5086)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1994"; volume: "137"; pages: "751-7"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(704..1262)
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus
                     nidulans trpC gene"
     CDS             complement(1434..2456)
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(2461..2817)
                     /label=trpC promoter
                     /note="promoter for Aspergillus nidulans trpC"
     primer_bind     complement(2865..2881)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2889..2905)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2913..2943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2958..2979)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4029..4886)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4887..4991)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt
      481 gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc
      541 cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc
      601 ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc
      661 ggtgatgccg gccacgatgc gtccggcgta gaggatcctc tagaaagaag gattacctct
      721 aaacaagtgt acctgtgcat tctgggtaaa cgactcatag gagagttgta aaaaagtttc
      781 ggccggcgta ttgggtgtta cggagcattc actaggcaac catggttact attgtatacc
      841 atcttagtag gaatgatttc gaggtttata cctacgatga atgtgtgtcc tgtaggcttg
      901 agagttcaag gaagaaacat gcaattatct ttgcgaaccc agggctggtg acggaatttt
      961 catagtcaag ctatcagagt aaagaagagg agcatgtcaa agtacaatta gagacaaata
     1021 tatagtcgcg tggagccaag agcggattcc tcagtctcgt aggtctcttg acgaccgttg
     1081 atctgcttga tctcgtctcc cgaaaatgaa aatagctctg ctaagctatt cttctcttcg
     1141 ccggagcctg aaggcgttac taggttgcag tcaatgcatt aatgcattgc agatgagctg
     1201 tatctggaag aggtaaaccc gaaaacgcgt tttattcttg ttgacatgga gctattaaat
     1261 cactagaagg cactctttgc tgcttggaca aatgaacgta tcttatcgag atcctgaaca
     1321 ccatttgtct caactccgga gctgacatcg acaccaacga tcttatatcc agattcgtca
     1381 agctgtttga tgatttcagt aacgttaagt ggatccggtc ggcatctact ctattccttt
     1441 gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca
     1501 tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg
     1561 gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca
     1621 accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg gagccgcggc
     1681 gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc
     1741 aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc
     1801 tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg
     1861 aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca
     1921 tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac
     1981 acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct
     2041 tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc
     2101 atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac
     2161 gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg
     2221 tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct
     2281 ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg
     2341 aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg
     2401 aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatcg
     2461 atgcttgggt agaataggta agtcagattg aatctgaaat aaagggagga agggcgaact
     2521 taagaaggta tgaccgggtc gttcacttac cttgcttgac aaacgcacaa gttatcgtgc
     2581 accaagcagc agatgataat aatgtcctcg ttcctgtctg ctaataagag tcacacttcg
     2641 agcgccgccg ctactgctta caagtgggct gatctgacca gttgcctaaa tgaaccatct
     2701 tgtcaaacga cacaaatttt gtgatccgcc tggacgacta aaccaaaata gcattgatgt
     2761 gttgacctcc actagctcca gccaagccca aaaatgctcc ttcaatatca tcttctgtcg
     2821 actctagagg atccccgggt accgagctcg aattcgtaat catggtcata gctgtttcct
     2881 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     2941 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3001 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3061 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4321 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4621 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
