Basic Vector Information
YEp353 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YEp353 vector Sequence
LOCUS YEp353. 7944 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast episomal vector with a URA3 marker for construction of lacZ fusions. For other reading frames, use YEp354 or YEp355. ACCESSION . VERSION . KEYWORDS YEp353. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7944) AUTHORS Myers AM, Tzagoloff A, Kinney DM, Lusty CJ. TITLE Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. JOURNAL Gene 1986;45:299-310. PUBMED 3026915 REFERENCE 2 (bases 1 to 7944) TITLE Direct Submission REFERENCE 3 (bases 1 to 7944) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1986"; volume: "45"; pages: "299-310" COMMENT SGRef: number: 2; type: "Journal Article" COMMENT The GenBank sequence was edited at base 3044 to eliminate a second EcoRI site. FEATURES Location/Qualifiers source 1..7944 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..36 /label=MCS /note="MCS" /note="multiple cloning site" CDS 45..3092 /label=lacZ /note="beta-galactosidase" CDS complement(3293..4093) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(4094..4304) /label=URA3 promoter rep_origin complement(4541..5705) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 5987..6091 /label=AmpR promoter CDS 6092..6949 /label=AmpR /note="beta-lactamase" rep_origin 7123..7711 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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