YEp353 vector (V010187)

Basic Vector Information

      • Vector Name:
      • YEp353
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 7944 bp
      • Type:
      • Yeast Plasmids
      • Source/Author:
      • Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
      • Copy Number:
      • High copy number

YEp353 vector Vector Map

YEp3537944 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900720075007800MCSlacZURA3URA3 promoter2u oriAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YEp353 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       YEp353.        7944 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. For other reading frames, use YEp354 or YEp355.
ACCESSION   .
VERSION     .
KEYWORDS    YEp353.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7944)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The GenBank sequence was edited at base 3044 to eliminate a second 
            EcoRI site.
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             45..3092
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3293..4093)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4094..4304)
                     /label=URA3 promoter
     rep_origin      complement(4541..5705)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5987..6091
                     /label=AmpR promoter
     CDS             6092..6949
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7123..7711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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