LOCUS       YEp353.        7944 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. For other reading frames, use YEp354 or YEp355.
ACCESSION   .
VERSION     .
KEYWORDS    YEp353
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7944)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7944)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     The GenBank sequence was edited at base 3044 to eliminate a second 
            EcoRI site.
FEATURES             Location/Qualifiers
     source          1..7944
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             45..3092
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3293..4093)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4094..4304)
                     /label=URA3 promoter
     rep_origin      complement(4541..5705)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5987..6091
                     /label=AmpR promoter
     CDS             6092..6949
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7123..7711
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttgcga tcccgccgtc gttttacaac
       61 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
      121 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
      181 gcctgaatgg cgaatggcgc tttgcctggt ttccggcacc agaagcggtg ccggaaagct
      241 ggctggagtg cgatcttcct gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc
      301 acggttacga tgcgcccatc tacaccaacg taacctatcc cattacggtc aatccgccgt
      361 ttgttcccac ggagaatccg acgggttgtt actcgctcac atttaatgtt gatgaaagct
      421 ggctacagga aggccagacg cgaattattt ttgatggcgt taactcggcg tttcatctgt
      481 ggtgcaacgg gcgctgggtc ggttacggcc aggacagtcg tttgccgtct gaatttgacc
      541 tgagcgcatt tttacgcgcc ggagaaaacc gcctcgcggt gatggtgctg cgttggagtg
      601 acggcagtta tctggaagat caggatatgt ggcggatgag cggcattttc cgtgacgtct
      661 cgttgctgca taaaccgact acacaaatca gcgatttcca tgttgccact cgctttaatg
      721 atgatttcag ccgcgctgta ctggaggctg aagttcagat gtgcggcgag ttgcgtgact
      781 acctacgggt aacagtttct ttatggcagg gtgaaacgca ggtcgccagc ggcaccgcgc
      841 ctttcggcgg tgaaattatc gatgagcgtg gtggttatgc cgatcgcgtc acactacgtc
      901 tgaacgtcga aaacccgaaa ctgtggagcg ccgaaatccc gaatctctat cgtgcggtgg
      961 ttgaactgca caccgccgac ggcacgctga ttgaagcaga agcctgcgat gtcggtttcc
     1021 gcgaggtgcg gattgaaaat ggtctgctgc tgctgaacgg caagccgttg ctgattcgag
     1081 gcgttaaccg tcacgagcat catcctctgc atggtcaggt catggatgag cagacgatgg
     1141 tgcaggatat cctgctgatg aagcagaaca actttaacgc cgtgcgctgt tcgcattatc
     1201 cgaaccatcc gctgtggtac acgctgtgcg accgctacgg cctgtatgtg gtggatgaag
     1261 ccaatattga aacccacggc atggtgccaa tgaatcgtct gaccgatgat ccgcgctggc
     1321 taccggcgat gagcgaacgc gtaacgcgaa tggtgcagcg cgatcgtaat cacccgagtg
     1381 tgatcatctg gtcgctgggg aatgaatcag gccacggcgc taatcacgac gcgctgtatc
     1441 gctggatcaa atctgtcgat ccttcccgcc cggtgcagta tgaaggcggc ggagccgaca
     1501 ccacggccac cgatattatt tgcccgatgt acgcgcgcgt ggatgaagac cagcccttcc
     1561 cggctgtgcc gaaatggtcc atcaaaaaat ggctttcgct acctggagag acgcgcccgc
     1621 tgatcctttg cgaatacgcc cacgcgatgg gtaacagtct tggcggtttc gctaaatact
     1681 ggcaggcgtt tcgtcagtat ccccgtttac agggcggctt cgtctgggac tgggtggatc
     1741 agtcgctgat taaatatgat gaaaacggca acccgtggtc ggcttacggc ggtgattttg
     1801 gcgatacgcc gaacgatcgc cagttctgta tgaacggtct ggtctttgcc gaccgcacgc
     1861 cgcatccagc gctgacggaa gcaaaacacc agcagcagtt tttccagttc cgtttatccg
     1921 ggcaaaccat cgaagtgacc agcgaatacc tgttccgtca tagcgataac gagctcctgc
     1981 actggatggt ggcgctggat ggtaagccgc tggcaagcgg tgaagtgcct ctggatgtcg
     2041 ctccacaagg taaacagttg attgaactgc ctgaactacc gcagccggag agcgccgggc
     2101 aactctggct cacagtacgc gtagtgcaac cgaacgcgac cgcatggtca gaagccgggc
     2161 acatcagcgc ctggcagcag tggcgtctgg cggaaaacct cagtgtgacg ctccccgccg
     2221 cgtcccacgc catcccgcat ctgaccacca gcgaaatgga tttttgcatc gagctgggta
     2281 ataagcgttg gcaatttaac cgccagtcag gctttctttc acagatgtgg attggcgata
     2341 aaaaacaact gctgacgccg ctgcgcgatc agttcacccg tgcaccgctg gataacgaca
     2401 ttggcgtaag tgaagcgacc cgcattgacc ctaacgcctg ggtcgaacgc tggaaggcgg
     2461 cgggccatta ccaggccgaa gcagcgttgt tgcagtgcac ggcagataca cttgctgatg
     2521 cggtgctgat tacgaccgct cacgcgtggc agcatcaggg gaaaacctta tttatcagcc
     2581 ggaaaaccta ccggattgat ggtagtggtc aaatggcgat taccgttgat gttgaagtgg
     2641 cgagcgatac accgcatccg gcgcggattg gcctgaactg ccagctggcg caggtagcag
     2701 agcgggtaaa ctggctcgga ttagggccgc aagaaaacta tcccgaccgc cttactgccg
     2761 cctgttttga ccgctgggat ctgccattgt cagacatgta taccccgtac gtcttcccga
     2821 gcgaaaacgg tctgcgctgc gggacgcgcg aattgaatta tggcccacac cagtggcgcg
     2881 gcgacttcca gttcaacatc agccgctaca gtcaacagca actgatggaa accagccatc
     2941 gccatctgct gcacgcggaa gaaggcacat ggctgaatat cgacggtttc catatgggga
     3001 ttggtggcga cgactcctgg agcccgtcag tatcggcgga attacagctg agcgccggtc
     3061 gctaccatta ccagttggtc tggtgtcaaa aataataata accgggcagg ccatgtctgc
     3121 ccgtatttcg cgtaaggaaa tccattatgt actatttcgc ctgatgcggt attttctcct
     3181 tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat taaattgaag
     3241 ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt tagttttgct
     3301 ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca ccctctacct
     3361 tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca gatcctgtag
     3421 agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg tcatctaaac
     3481 ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc atgtctcttt
     3541 gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca gtacccttag
     3601 tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc aaaaggcctc
     3661 taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata cctgggccca
     3721 ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca cccgcagagt
     3781 actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag agtaaaaaat
     3841 tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa aaatcagtca
     3901 agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca actaactcca
     3961 gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc ttttcgtgca
     4021 tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt tccttatatg
     4081 tagctttcga catgatttat cttcgttttt tgttctgtgc agttgggtta agaatactgg
     4141 gcaatttcat gtttcttcaa cactacatat gcgtatatat accaatctaa gtctgtgctc
     4201 cttccttcgt tcttccttct gttcggagat taccgaatca aaaaaatttc aaagaaaccg
     4261 aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaat tgaaaagcta cttgttaccc
     4321 atcattgaat tttgaacatc cgaacctggg agttttccct gaaacagata gtatatttga
     4381 acctgtataa taatatatag tctagcgctt tacggaagac aatgtatgta tttcggttcc
     4441 tggagaaact attgcatcta ttgcataggt aatcttgcac gtcgcatccc cggttcattt
     4501 tctgcgtttc catcttgcac ttcaatagca tatctttgtt aacgaagcat ctgtgcttca
     4561 ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     4621 catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa gaatctgtgc
     4681 ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg
     4741 agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca acaaagaatc
     4801 tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc taacaaagca
     4861 tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg ataacttttt
     4921 gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt ctcttccata
     4981 aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc gggtgcattt
     5041 tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg catactttgt
     5101 gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga acggtttctt
     5161 ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt gttttcgatt
     5221 cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact agagataaac
     5281 ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt ggatgggtag
     5341 gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag caatgtttgt
     5401 ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt ttggtttttt
     5461 gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc ctatactttc
     5521 tagctagaga ataggaactt cggaatagga acttcaaagc gtttccgaaa acgagcgctt
     5581 ccgaaaatgc aacgcgagct gcgcacatac agctcactgt tcacgtcgca cctatatctg
     5641 cgtgttgcct gtatatatat atacatgaga agaacggcat agtgcgtgtt tatgcttaaa
     5701 tgcgttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     5761 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5821 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5881 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5941 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6001 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6061 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6121 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6181 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6241 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6301 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6361 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     6421 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     6481 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     6541 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     6601 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     6661 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     6721 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     6781 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     6841 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     6901 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     6961 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7021 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7081 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7141 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7201 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7261 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7321 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7381 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     7441 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     7501 cctacagcgt gagcattgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     7561 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     7621 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     7681 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     7741 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     7801 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     7861 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     7921 cgcgcgttgg ccgattcatt cccg
//
