Basic Vector Information
- Vector Name:
- YEp354
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7942 bp
- Type:
- Yeast Plasmids
- Replication origin:
- ori
- Host:
- Yeast
- Source/Author:
- Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
- Copy Number:
- High copy number
- Promoter:
- URA3
YEp354 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
YEp354 vector Sequence
LOCUS YEp354. 7942 bp DNA circular SYN 01-JAN-1980 DEFINITION Yeast episomal vector with a URA3 marker for construction of lacZ fusions. For other reading frames, use YEp353 or YEp355. ACCESSION . VERSION . KEYWORDS YEp354. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7942) AUTHORS Myers AM, Tzagoloff A, Kinney DM, Lusty CJ. TITLE Yeast shuttle and integrative vectors with multiple cloning sites suitable for construction of lacZ fusions. JOURNAL Gene 1986;45:299-310. PUBMED 3026915 REFERENCE 2 (bases 1 to 7942) TITLE Direct Submission REFERENCE 3 (bases 1 to 7942) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; date: "1986"; volume: "45"; pages: "299-310" COMMENT SGRef: number: 2; type: "Journal Article" FEATURES Location/Qualifiers source 1..7942 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..36 /label=MCS /note="MCS" /note="multiple cloning site" CDS 43..3090 /label=lacZ /note="beta-galactosidase" CDS complement(3291..4091) /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" promoter complement(4092..4302) /label=URA3 promoter rep_origin complement(4539..5703) /direction=LEFT /label=2u ori /note="yeast 2u plasmid origin of replication" promoter 5985..6089 /label=AmpR promoter CDS 6090..6947 /label=AmpR /note="beta-lactamase" rep_origin 7121..7709 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
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