LOCUS       YEp354.        7942 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. For other reading frames, use YEp353 or YEp355.
ACCESSION   .
VERSION     .
KEYWORDS    YEp354
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7942)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7942)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7942)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             43..3090
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3291..4091)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4092..4302)
                     /label=URA3 promoter
     rep_origin      complement(4539..5703)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5985..6089
                     /label=AmpR promoter
     CDS             6090..6947
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7121..7709
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttgctc ccgccgtcgt tttacaacgt
       61 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      121 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      181 ctgaatggcg aatggcgctt tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg
      241 ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac
      301 ggttacgatg cgcccatcta caccaacgta acctatccca ttacggtcaa tccgccgttt
      361 gttcccacgg agaatccgac gggttgttac tcgctcacat ttaatgttga tgaaagctgg
      421 ctacaggaag gccagacgcg aattattttt gatggcgtta actcggcgtt tcatctgtgg
      481 tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt tgccgtctga atttgacctg
      541 agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga tggtgctgcg ttggagtgac
      601 ggcagttatc tggaagatca ggatatgtgg cggatgagcg gcattttccg tgacgtctcg
      661 ttgctgcata aaccgactac acaaatcagc gatttccatg ttgccactcg ctttaatgat
      721 gatttcagcc gcgctgtact ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac
      781 ctacgggtaa cagtttcttt atggcagggt gaaacgcagg tcgccagcgg caccgcgcct
      841 ttcggcggtg aaattatcga tgagcgtggt ggttatgccg atcgcgtcac actacgtctg
      901 aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga atctctatcg tgcggtggtt
      961 gaactgcaca ccgccgacgg cacgctgatt gaagcagaag cctgcgatgt cggtttccgc
     1021 gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca agccgttgct gattcgaggc
     1081 gttaaccgtc acgagcatca tcctctgcat ggtcaggtca tggatgagca gacgatggtg
     1141 caggatatcc tgctgatgaa gcagaacaac tttaacgccg tgcgctgttc gcattatccg
     1201 aaccatccgc tgtggtacac gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc
     1261 aatattgaaa cccacggcat ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta
     1321 ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg
     1381 atcatctggt cgctggggaa tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc
     1441 tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc
     1501 acggccaccg atattatttg cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg
     1561 gctgtgccga aatggtccat caaaaaatgg ctttcgctac ctggagagac gcgcccgctg
     1621 atcctttgcg aatacgccca cgcgatgggt aacagtcttg gcggtttcgc taaatactgg
     1681 caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg tctgggactg ggtggatcag
     1741 tcgctgatta aatatgatga aaacggcaac ccgtggtcgg cttacggcgg tgattttggc
     1801 gatacgccga acgatcgcca gttctgtatg aacggtctgg tctttgccga ccgcacgccg
     1861 catccagcgc tgacggaagc aaaacaccag cagcagtttt tccagttccg tttatccggg
     1921 caaaccatcg aagtgaccag cgaatacctg ttccgtcata gcgataacga gctcctgcac
     1981 tggatggtgg cgctggatgg taagccgctg gcaagcggtg aagtgcctct ggatgtcgct
     2041 ccacaaggta aacagttgat tgaactgcct gaactaccgc agccggagag cgccgggcaa
     2101 ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg catggtcaga agccgggcac
     2161 atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg
     2221 tcccacgcca tcccgcatct gaccaccagc gaaatggatt tttgcatcga gctgggtaat
     2281 aagcgttggc aatttaaccg ccagtcaggc tttctttcac agatgtggat tggcgataaa
     2341 aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg caccgctgga taacgacatt
     2401 ggcgtaagtg aagcgacccg cattgaccct aacgcctggg tcgaacgctg gaaggcggcg
     2461 ggccattacc aggccgaagc agcgttgttg cagtgcacgg cagatacact tgctgatgcg
     2521 gtgctgatta cgaccgctca cgcgtggcag catcagggga aaaccttatt tatcagccgg
     2581 aaaacctacc ggattgatgg tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg
     2641 agcgatacac cgcatccggc gcggattggc ctgaactgcc agctggcgca ggtagcagag
     2701 cgggtaaact ggctcggatt agggccgcaa gaaaactatc ccgaccgcct tactgccgcc
     2761 tgttttgacc gctgggatct gccattgtca gacatgtata ccccgtacgt cttcccgagc
     2821 gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc
     2881 gacttccagt tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc
     2941 catctgctgc acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt
     3001 ggtggcgacg actcctggag cccgtcagta tcggcggaat tacagctgag cgccggtcgc
     3061 taccattacc agttggtctg gtgtcaaaaa taataataac cgggcaggcc atgtctgccc
     3121 gtatttcgcg taaggaaatc cattatgtac tatttcgcct gatgcggtat tttctcctta
     3181 cgcatctgtg cggtatttca caccgcatag ggtaataact gatataatta aattgaagct
     3241 ctaatttgtg agtttagtat acatgcattt acttataata cagtttttta gttttgctgg
     3301 ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt aacgttcacc ctctacctta
     3361 gcatcccttc cctttgcaaa tagtcctctt ccaacaataa taatgtcaga tcctgtagag
     3421 accacatcat ccacggttct atactgttga cccaatgcgt ctcccttgtc atctaaaccc
     3481 acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc ttccacccat gtctctttga
     3541 gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa tgtcaacagt acccttagta
     3601 tattctccag tagataggga gcccttgcat gacaattctg ctaacatcaa aaggcctcta
     3661 ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc taacaatacc tgggcccacc
     3721 acaccgtgtg cattcgtaat gtctgcccat tctgctattc tgtatacacc cgcagagtac
     3781 tgcaatttga ctgtattacc aatgtcagca aattttctgt cttcgaagag taaaaaattg
     3841 tacttggcgg ataatgcctt tagcggctta actgtgccct ccatggaaaa atcagtcaag
     3901 atatccacat gtgtttttag taaacaaatt ttgggaccta atgcttcaac taactccagt
     3961 aattccttgg tggtacgaac atccaatgaa gcacacaagt ttgtttgctt ttcgtgcatg
     4021 atattaaata gcttggcagc aacaggacta ggatgagtag cagcacgttc cttatatgta
     4081 gctttcgaca tgatttatct tcgttttttg ttctgtgcag ttgggttaag aatactgggc
     4141 aatttcatgt ttcttcaaca ctacatatgc gtatatatac caatctaagt ctgtgctcct
     4201 tccttcgttc ttccttctgt tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa
     4261 atcaaaaaaa agaataaaaa aaaaatgatg aattgaattg aaaagctact tgttacccat
     4321 cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt atatttgaac
     4381 ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt tcggttcctg
     4441 gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg gttcattttc
     4501 tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct gtgcttcatt
     4561 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
     4621 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
     4681 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
     4741 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
     4801 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
     4861 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
     4921 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
     4981 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
     5041 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
     5101 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
     5161 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
     5221 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
     5281 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
     5341 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
     5401 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
     5461 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
     5521 gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac gagcgcttcc
     5581 gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc tatatctgcg
     5641 tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta tgcttaaatg
     5701 cgttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5761 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5821 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5881 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     5941 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6001 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6061 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6121 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6181 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6241 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6301 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6361 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6421 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6481 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6541 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6601 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6661 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6721 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6781 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6841 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6901 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6961 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7021 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7081 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7141 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7201 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7261 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7321 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7381 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7441 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7501 tacagcgtga gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7561 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7621 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7681 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7741 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7801 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7861 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7921 cgcgttggcc gattcattcc cg
//
