YEp355 vector (V010185)

MSDS

Basic Vector Information

Vector Name:: YEp355

Antibiotic Resistance:: Ampicillin

Length:: 7943 bp

Type:: Yeast Plasmids

Replication origin:: ori

Host:: Yeast

Source/Author:: Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.

Copy Number:: High copy number

Promoter:: URA3

YEp355 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

YEp355 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       YEp355.        7943 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. For other reading frames, use YEp353 or YEp354.
ACCESSION   .
VERSION     .
KEYWORDS    YEp355.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7943)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7943)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             44..3091
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3292..4092)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4093..4303)
                     /label=URA3 promoter
     rep_origin      complement(4540..5704)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5986..6090
                     /label=AmpR promoter
     CDS             6091..6948
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7122..7710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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