LOCUS       YEp355.        7943 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Yeast episomal vector with a URA3 marker for construction of lacZ 
            fusions. For other reading frames, use YEp353 or YEp354.
ACCESSION   .
VERSION     .
KEYWORDS    YEp355
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Myers AM, Tzagoloff A, Kinney DM, Lusty CJ.
  TITLE     Yeast shuttle and integrative vectors with multiple cloning sites 
            suitable for construction of lacZ fusions.
  JOURNAL   Gene 1986;45:299-310.
  PUBMED    3026915
REFERENCE   2  (bases 1 to 7943)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7943)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1986"; volume: "45"; pages: "299-310"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7943
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..36
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             44..3091
                     /label=lacZ
                     /note="beta-galactosidase"
     CDS             complement(3292..4092)
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     promoter        complement(4093..4303)
                     /label=URA3 promoter
     rep_origin      complement(4540..5704)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        5986..6090
                     /label=AmpR promoter
     CDS             6091..6948
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7122..7710
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gaattcccgg ggatccgtcg acctgcagcc aagcttcgat cccgccgtcg ttttacaacg
       61 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
      121 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
      181 cctgaatggc gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg
      241 gctggagtgc gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca
      301 cggttacgat gcgcccatct acaccaacgt aacctatccc attacggtca atccgccgtt
      361 tgttcccacg gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg
      421 gctacaggaa ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg
      481 gtgcaacggg cgctgggtcg gttacggcca ggacagtcgt ttgccgtctg aatttgacct
      541 gagcgcattt ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gttggagtga
      601 cggcagttat ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc
      661 gttgctgcat aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga
      721 tgatttcagc cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta
      781 cctacgggta acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc
      841 tttcggcggt gaaattatcg atgagcgtgg tggttatgcc gatcgcgtca cactacgtct
      901 gaacgtcgaa aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt
      961 tgaactgcac accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg
     1021 cgaggtgcgg attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg
     1081 cgttaaccgt cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt
     1141 gcaggatatc ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc
     1201 gaaccatccg ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc
     1261 caatattgaa acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct
     1321 accggcgatg agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt
     1381 gatcatctgg tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg
     1441 ctggatcaaa tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac
     1501 cacggccacc gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc
     1561 ggctgtgccg aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct
     1621 gatcctttgc gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg
     1681 gcaggcgttt cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca
     1741 gtcgctgatt aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg
     1801 cgatacgccg aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc
     1861 gcatccagcg ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg
     1921 gcaaaccatc gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca
     1981 ctggatggtg gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc
     2041 tccacaaggt aaacagttga ttgaactgcc tgaactaccg cagccggaga gcgccgggca
     2101 actctggctc acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca
     2161 catcagcgcc tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tccccgccgc
     2221 gtcccacgcc atcccgcatc tgaccaccag cgaaatggat ttttgcatcg agctgggtaa
     2281 taagcgttgg caatttaacc gccagtcagg ctttctttca cagatgtgga ttggcgataa
     2341 aaaacaactg ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat
     2401 tggcgtaagt gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc
     2461 gggccattac caggccgaag cagcgttgtt gcagtgcacg gcagatacac ttgctgatgc
     2521 ggtgctgatt acgaccgctc acgcgtggca gcatcagggg aaaaccttat ttatcagccg
     2581 gaaaacctac cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtggc
     2641 gagcgataca ccgcatccgg cgcggattgg cctgaactgc cagctggcgc aggtagcaga
     2701 gcgggtaaac tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgccgc
     2761 ctgttttgac cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag
     2821 cgaaaacggt ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg
     2881 cgacttccag ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg
     2941 ccatctgctg cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat
     3001 tggtggcgac gactcctgga gcccgtcagt atcggcggaa ttacagctga gcgccggtcg
     3061 ctaccattac cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc
     3121 cgtatttcgc gtaaggaaat ccattatgta ctatttcgcc tgatgcggta ttttctcctt
     3181 acgcatctgt gcggtatttc acaccgcata gggtaataac tgatataatt aaattgaagc
     3241 tctaatttgt gagtttagta tacatgcatt tacttataat acagtttttt agttttgctg
     3301 gccgcatctt ctcaaatatg cttcccagcc tgcttttctg taacgttcac cctctacctt
     3361 agcatccctt ccctttgcaa atagtcctct tccaacaata ataatgtcag atcctgtaga
     3421 gaccacatca tccacggttc tatactgttg acccaatgcg tctcccttgt catctaaacc
     3481 cacaccgggt gtcataatca accaatcgta accttcatct cttccaccca tgtctctttg
     3541 agcaataaag ccgataacaa aatctttgtc gctcttcgca atgtcaacag tacccttagt
     3601 atattctcca gtagataggg agcccttgca tgacaattct gctaacatca aaaggcctct
     3661 aggttccttt gttacttctt ctgccgcctg cttcaaaccg ctaacaatac ctgggcccac
     3721 cacaccgtgt gcattcgtaa tgtctgccca ttctgctatt ctgtatacac ccgcagagta
     3781 ctgcaatttg actgtattac caatgtcagc aaattttctg tcttcgaaga gtaaaaaatt
     3841 gtacttggcg gataatgcct ttagcggctt aactgtgccc tccatggaaa aatcagtcaa
     3901 gatatccaca tgtgttttta gtaaacaaat tttgggacct aatgcttcaa ctaactccag
     3961 taattccttg gtggtacgaa catccaatga agcacacaag tttgtttgct tttcgtgcat
     4021 gatattaaat agcttggcag caacaggact aggatgagta gcagcacgtt ccttatatgt
     4081 agctttcgac atgatttatc ttcgtttttt gttctgtgca gttgggttaa gaatactggg
     4141 caatttcatg tttcttcaac actacatatg cgtatatata ccaatctaag tctgtgctcc
     4201 ttccttcgtt cttccttctg ttcggagatt accgaatcaa aaaaatttca aagaaaccga
     4261 aatcaaaaaa aagaataaaa aaaaaatgat gaattgaatt gaaaagctac ttgttaccca
     4321 tcattgaatt ttgaacatcc gaacctggga gttttccctg aaacagatag tatatttgaa
     4381 cctgtataat aatatatagt ctagcgcttt acggaagaca atgtatgtat ttcggttcct
     4441 ggagaaacta ttgcatctat tgcataggta atcttgcacg tcgcatcccc ggttcatttt
     4501 ctgcgtttcc atcttgcact tcaatagcat atctttgtta acgaagcatc tgtgcttcat
     4561 tttgtagaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc
     4621 atttttacag aacagaaatg caacgcgaaa gcgctatttt accaacgaag aatctgtgct
     4681 tcatttttgt aaaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     4741 gctgcatttt tacagaacag aaatgcaacg cgagagcgct attttaccaa caaagaatct
     4801 atacttcttt tttgttctac aaaaatgcat cccgagagcg ctatttttct aacaaagcat
     4861 cttagattac tttttttctc ctttgtgcgc tctataatgc agtctcttga taactttttg
     4921 cactgtaggt ccgttaaggt tagaagaagg ctactttggt gtctattttc tcttccataa
     4981 aaaaagcctg actccacttc ccgcgtttac tgattactag cgaagctgcg ggtgcatttt
     5041 ttcaagataa aggcatcccc gattatattc tataccgatg tggattgcgc atactttgtg
     5101 aacagaaagt gatagcgttg atgattcttc attggtcaga aaattatgaa cggtttcttc
     5161 tattttgtct ctatatacta cgtataggaa atgtttacat tttcgtattg ttttcgattc
     5221 actctatgaa tagttcttac tacaattttt ttgtctaaag agtaatacta gagataaaca
     5281 taaaaaatgt agaggtcgag tttagatgca agttcaagga gcgaaaggtg gatgggtagg
     5341 ttatataggg atatagcaca gagatatata gcaaagagat acttttgagc aatgtttgtg
     5401 gaagcggtat tcgcaatatt ttagtagctc gttacagtcc ggtgcgtttt tggttttttg
     5461 aaagtgcgtc ttcagagcgc ttttggtttt caaaagcgct ctgaagttcc tatactttct
     5521 agctagagaa taggaacttc ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc
     5581 cgaaaatgca acgcgagctg cgcacataca gctcactgtt cacgtcgcac ctatatctgc
     5641 gtgttgcctg tatatatata tacatgagaa gaacggcata gtgcgtgttt atgcttaaat
     5701 gcgttatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     5761 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     5821 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     5881 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     5941 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     6001 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     6061 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     6121 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     6181 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     6241 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     6301 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     6361 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     6421 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     6481 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     6541 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     6601 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     6661 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     6721 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     6781 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     6841 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     6901 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     6961 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     7021 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     7081 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     7141 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     7201 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     7261 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     7321 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     7381 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     7441 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     7501 ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     7561 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     7621 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     7681 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     7741 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     7801 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     7861 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     7921 gcgcgttggc cgattcattc ccg
//
